| 用HI和ELISA方法检测SPF鸡血清和蛋黄中ND抗体的比较 |
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| 引用本文： | 周广驰,魏艳华,赵成莹,袁静,孙晓军,欧阳文军.用HI和ELISA方法检测SPF鸡血清和蛋黄中ND抗体的比较[J].山东家禽,2012(8):9-11. |
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| 作者姓名： | 周广驰 魏艳华 赵成莹 袁静 孙晓军 欧阳文军 |
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| 作者单位： | [1]山东省聊城市东昌府区畜牧局,山东聊城252000 [2]山东省农业科学院家禽研究所,山东济南250023 |
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| 基金项目： | 山东省自然科学基金项目（zR2010CQ044）和山东省科技发展计划项目（2009GG10009006）. |
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| 摘 要： | 分别用血凝抑制试验（HI）和酶联免疫吸附试验（ELISA）检测42周和48周SPF鸡血清、以及同期SPF鸡蛋蛋黄中新城疫（ND）抗体。结果表明，两种方法检测的120份血清中均无ND抗体，呈阴性；120枚SPF鸡蛋蛋黄检测，ELISA方法检测结果全为阴性；HI方法检测结果25份阳性，差异较为明显。
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| 关 键 词： | 血凝抑制试验 酶联免疫试验 SPF鸡血清和蛋黄 新城疫抗体 |
Compare HI and ELISA to diagnose ND antibody level in SPF chicken serum and egg yolk |
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| Institution: | ZHOU Guang-chi, WEI Yan-hua, ZHAO Cheng-ying, YUAN-Jing, SUN Xiao-jun,OUYANG Wen-jun (1.Shangdong Province Liaocheng City Dongchangfu District Husbandry and veterinary Bureau, Liaocheng, Shangdong 252000 ; 2.Shangdong Province Institute of Agricultural Sciences Department of Poultry Science, Jinan , 250023) |
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| Abstract: | hemagglutination inhibition （HI） and Enzyme-linked immunosorbent assay （ELISA） were utilized to test Newcastle Disease （ND） antibody in the serum and yolk from 42 weeks and 48 weeks old SPF chicken and their simultaneous eggs. The result indicates that no ND antibody are detected among the 120 serum samples by both methods and all are negative while in the 120 yolk samples, results from ELISA tests are all negative and HI results include 25 positive, where significant difference is observed. |
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| Keywords: | hemagglutination inhibition ELISA SPF chicken serum and yolk NewcastleDisease Antibody |
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