| 乳酸菌复合系SFC-2增菌培养基的优化 |
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| 引用本文： | 姜飞,李翔国,刘海峰,全炳武,崔宗均. 乳酸菌复合系SFC-2增菌培养基的优化[J]. 延边大学农学学报, 2013, 0(1): 11-17 |
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| 作者姓名： | 姜飞 李翔国 刘海峰 全炳武 崔宗均 |
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| 作者单位： | 延边大学农学院农学系;中国农业大学农学与生物技术学院 |
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| 基金项目： | 国家公益性(农业)科研专项(200803033-B0502) |
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| 摘 要： | 通过对乳酸菌复合系SFC-2培养基的组分进行响应面优化，从而得出SFC-2优化培养基配方为白砂糖10g／L、蛋白胨31．36g／L、牛肉膏15．57g／L、酵母膏10g／L、乙酸钠7．3g／L、柠檬酸三铵4g／L、KzHP044g／L、MgS04·7H200．58g／L、MnSO4·H200．25g／L。SFC-2在此培养基中经30℃，16h培养，其活菌数可达到4．0×10^8cfu／mL，较MRS-S中（8．2×10^7cfu／mL）提高近5倍。
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| 关 键 词： | 乳酸菌复合系SFC-2 响应面优化法 培养基 |
Optimization of the enrichment medium for LAB community SFC-2 |
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| JIANG Fei,LI Xiang-guo,LIU Hai-feng,QUAN Bing-wu,CUI Zong-jun. Optimization of the enrichment medium for LAB community SFC-2[J]. Journal of Agricultural Science Yanbian University, 2013, 0(1): 11-17 |
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| Authors: | JIANG Fei LI Xiang-guo LIU Hai-feng QUAN Bing-wu CUI Zong-jun |
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| Affiliation: | 1.Agricultural Department,Agricultural College of Yanbian University,Yanji Jilin 133002 China; 2.Agricultural and Biotechnology College of China Agricultural University,Beijing 100094,China) |
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| Abstract: | The medium components for LAB community SFC-2 were optimized by response surface meth- odology,which contained white granulated sugar 10 g/L,peptone 31.36 g/L,beef extract 15.57 g/L,yeast extract 10 g/L,sodium acetate 7.3 g/L,triammonium citrate 4 g/L,K2 HPO4 4 g/L,MgSO4 · 7H2O 0. 58 g/L,MnSO4 · H2O 0.25 g/L. After cultivation in optimization medium for 16 h at 30 ℃ ,the viable count of SFC-2 was 4.0 × 10a cfu/mL, which was about 5 times higher than that in MRS-S（8.2 × 107 cfu/mL）. |
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| Keywords: | LAB community SFC-2 response surface methodology medium |
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