EST-SSR based estimates on functional genetic variation in a barley (<Emphasis Type="Italic">Hordeum vulgare</Emphasis> L.) collection from Egypt |
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Authors: | Khaled F M Salem Rajeev K Varshney Marion S Röder Andreas Börner |
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Institution: | (1) Genetic Engineering and Biotechnology Research Institute (GEBRI), Menoufia University, P. O. Box 79, Sadat City, Egypt;(2) International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru-502324, Greater Hyderabad, India;(3) Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany |
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Abstract: | Genetic diversity was investigated in a set of 27 barley genotypes, originated from Egypt, using 23 expressed sequence tag
(EST)-derived simple sequence repeats (eSSRs), representatives of the seven barley chromosomes. Ninety-five alleles were detected
among all the barley accessions. For 22 polymorphic eSSRs, the number of alleles per locus varied from 2 to 12, with a mean
of 4.318 alleles per locus. The lowest and the largest number of alleles per locus among the seven homeologous groups was
observed in homeologous group 5H and 4H with 3.00 and 6.33, respectively. The gene diversity increased as the number of alleles
increased. Gene diversity for 22 ESTs loci varied from 0.137 for GBM1404 to 0.896 for GBM1015 with an average of 0.563. A
significant correlation coefficient between gene diversity and the number of alleles was high, r = 0.741 (P < 0.01). Cluster analysis was conducted based on eSSRs data to group the barley genotypes and to construct a dendrogram.
Four groups can be distinguished by truncating the dendrogram at gs value of 0.77. |
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