羊栖菜Sargasum_fusiforme原生质体的分离和培养

本研究多次获得原生质体，明确了酶解原生质体的内在因素与外在条件，诸如酶溶剂的种类及浓度，酶及组成，酶解温度及ｐＨ，采种藻时间及取用部位。分离的原生质体经初步培养，发现少数２分裂，最好的有成６分裂的细胞团，但尚未成株。研究结果为扩大培养打下了良好的基础。此外，还报道了用海洋细菌酶解的试验及意义

THE ISOLATION AND CULTURE OF PROTOPLASTS FROM SARGASSUM FUSIFORME ( PHAEOPHYTA, SARGASSACEAE)

Protoplasts from one species of brown alga Sargassum fusiforme (Harv.) Setchell had been obtained with the application of wall degrading enzymes.The internal factors and external conditions of the isolation of protoplasts ,such as kinds and concentrations of osmoregulated reagent,kinds and combinations of enzymes,isolated temperature and pH, time of material collections and part of thallus, are definited now. Having been cultured for 3 days, some protoplasts regenerated cell walls and divided into two cells. Six cell callus like cell mass was also obtained after being cultured for 6 days. These results are good basis for further culture in large scale. The application of marine bacteria on isolating vegetative cells of these alga is reported in this paper.