烟草青枯病内生拮抗菌的筛选、鉴定及其防效测定

 植物体内的大量微生物,在进化的过程中,由于长期生活在植物特定部位,因而与植物形成了一种特殊的关系,这些微生物在植物体内大量繁殖和扩散,有望成为生物防治有潜力的微生物。内生细菌是植物体内大量存在的微生物之一,具有在植株体内分布广、定殖能力强、防效好以及增殖和扩散快等优点,因而成为发展前景很好的植物病害生防菌。

Screening, identification and control efficacy of tobacco antagonistic endophytic bacteria against Ralstonia solanacearum

One hundred and sixty endophytic bacteria strains were isolated from healthy tobacco plants in severely diseased plots in Guiyang county,Hunan province.Three endophytic bacteria strains: H-1,H-9 and H-11 showed relatively strong antagonistic against Ralstonia solanacearum.Strain H-1 was identified as Bacillus subtilis,while strains H-9 and H-11 were identified as Bacillus brevis by their morphological,physiological and biochemical characteristics.Results from several experiment trials showed that the endophytic bacteria could reduce the disease incidence of tobacco.The control efficiency reached from 84.3% to 98.7%,it indicated that biocontrol with endophytic bacteria was an alternative and potential method to control tobacco bacterial disease.