陆地棉不同纤维发育突变体及其SSR指纹分析

 本研究对3组不同纤维发育突变体的近等基因系，利用SSR分子标记技术，进行了多态性分析及遗传相似性和聚类分析。3组近等基因系分别是：（1）陆地棉遗传标准系TM-1、无绒有絮突变体光子Nn、光子nn、H-154（n2）和毛子NN2-2；（2）无绒无絮突变体徐州142无絮和徐州142；（3）无绒无絮突变体新乡小吉无絮可能是中棉所12或豫棉4号的突变体。本文SSR分子标记和形态学鉴定表明新乡小吉无絮可能来源于豫棉4号，并从分子标记的角度表明了XZ142w突变体与野生型相比较，具有较多基因突变和遗传差异。研究还表明多态性SSR引物能将相似表现型的突变体聚在一起，这说明种质的表型变异度与SSR聚类分析结果间有很好的吻合度，因而，SSR标记技术是分析种质间遗传差异和遗传多样性的有效方法。

SSR Fingerprinting Analysis on Distinct Mutants of Fiber Development in Gossypium hisutum

The polymorphism and cluster analysis based on genetic similarity matrices of SSR markers were used to identify three groups of isogenic lines genetically distinct in fiber development. The three groups of isogenic mutants includes: (1) fuzzless-lint mutant GZNn, GZnn, n2, fuzzed-lint line NN2-2 and the upland cotton genetic standard line TM-1; (2) fuzzless-lintless mutant XZ142w and its wild type XZ142. (3) fuzzless-lintless mutant Xinxiang xiaojie wuxu (Xin) which maybe derived from CCRI No.12 or Yumian No.4. SSR markers and morphologic analysis indicated that the mutant fuzzless-lintless Xin maybe the mutant of Yumian No.4. The molecular marker analysis indicated that the mutant XZ142w possessed lots of mutant genes and genetic differences compared with that of the wild type. .Moreover, the results of research showed that the polymorphic SSR primers can groupe the mutants with similar phenotypes together. This indicated that the variability of germplasm phenotypes accorded with the cluster analysis of SSR markers. Therefore, SSR marker technology is one kind of effective techniques for analysis germplasm genetic differences and biodiversity.