苎麻叶片组织培养再生植株的研究

摘要：以中苎一号叶片为外植体，研究了外植体消毒时间、培养基、生长调节物质对苎麻品种中苎一号叶片愈伤诱导、分化不定芽和生根的影响。其结果表明：先用75%酒精消毒10秒，再用0.1%升汞灭菌8分钟，同时滴入1-2滴1% HCL效果最好。中苎一号最佳叶片愈伤组织诱导基本培养基为1/2MS;最佳叶片愈伤组织诱导培养基组合为:1/2MS+0.05mg/L TDZ+0.01mg/L IAA+0.03mg/L 2,4-D;最佳愈伤分化培养基为: 1/2MS+0.5mg/L TDZ+0.02mg/L 2,4-D+0.03mg/L IAA;最佳生根培养基为:1/2MS+0.02mg/L TDZ+O.O5mg/L NAA+0.02mg/L IAA, 形成了较为完善的组织培养再生体系，再生率达到了26%以上

Study on Regeneration Plants by tissue culture used Leaf Explant in Ramie

Abstract: Based on the leaf-blades of Ramie being for the outside plants body, this paper researches on the duration of disinfection, culture medium and the growth of adjustment materials which affect the leaves of Ramie’s callus induction, the occurrence of adventitious buds and rooting. The result shows that, firstly, using 75% of alcohol to have disinfection in 10 seconds; then, using 0.1% mercuric chloride to have sterilization in 8 minutes,dripping into 1-2 drop of 1% HCL at the same time, it is the optium way to cause effective result. The best leaves of Ramie’s callus induction basic culture medium is 1/2MS. The best leaves induction organization culture medium combination is that: 1/2MS+0.05mg/L TDZ+0.01mg/L IAA+0.03mg/L 2,4-D; The optium injury differentiation medium is that: 1/2MS+0.5mg/L TDZ+0.02mg/L 2,4-D+0.03mg/L IAA; The best rooting medium is that: 1/2MS+0.02mg/L TDZ+O.O5mg/L NAA+0.02mg/L IAA. Thus, it has formed the more perfect tissue culture regeneration system, regeneration rate has reached 26% the above.