猪圆环病毒2型PCR检测方法的建立及其应用

参照国内外已发表的猪圆环病毒2型（PCV-2）的ORF1的基因序列及其相关的PCR检测方法设计一对引物,扩增目的片段为493bp。通过对PCR检测方法的特异性、敏感性、重复性试验,建立了一种PCV-2的PCR检测方法。应用此方法对临床样品进行了检测,阳性检出率达51.18%。该方法具有快速、敏感、特异等优点,可用于PCV-2的检测和流行病学调查等。

Establishment and Application of PCR for Detection of PCV-2

According to the ORF1 gene sequence of PCV-2 published in GenBank, and the interrelated detect method of PCR, a pair of primers were designed, the motive fragment of amplification is 493bp. A PCR detective method was established by specificity,sensibility and reproducibility test. The method had some advantages such as fast,sensitive and specific. And it was considered to be the foundation for detecting and epidemiologic survey of PCV-2. Using the method to detect the clinic samples, the positive rate was 51.18%.