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High frequency of abnormal high molecular weight glutenin alleles in Chinese wheat landraces of the Yangtze-River region |
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| Authors: | Wei ZhengYanchun Peng Junhong MaRudi Appels Dongfa Sun Wujun Ma |
| Institution: | a Department of Agriculture, Huazhong Agriculture University, Wuhan, 430070 China b Western Australian Department of Agriculture & Food, Perth, WA 6150, Australia c Centre for Comparative Genomics, Murdoch University, Perth, WA 6150, Australia |
| Abstract: | A total of 485 common landraces of bread wheat were collected from the Yangtze-River region of China. Their high molecular weight glutenin subunit (HMW-GS) composition was analyzed by Matrix-assisted laser desorption/ionization time-of-flight Mass Spectrometry (MALDI-TOF-MS). Among all landraces tested, 453 were homogeneous for HMW-GS, 32 were heterogeneous, and 37 contained abnormal subunits. A total of 22 alleles were detected, including 3 at Glu-A1, 13 at Glu-B1 and 6 at Glu-D1, respectively. Higher variations occurred at the Glu-B1 locus compared with Glu-A1 and Glu-D1. Glu-A1c (74.0%), Glu-B1b (40.4%), Glu-D1a (84.9%) appeared to be the most frequent alleles at Glu-A1, Glu-B1 and Glu-D1, respectively. Two alleles ("null" and 1) at the Glu-A1 locus, three allele compositions (7 + 8, 7OE + 8, 7 + 9) at the Glu-B1 locus, and two (2 + 12 and 5 + 10) at the Glu-D1 locus appeared to be the common types in the 485 landraces. Sixteen new alleles represented by abnormal subunits were identified at the Glu-B1 and the Glu-D1 locus. |
| Keywords: | Yangtze-River region High molecular weight glutenin Wheat landraces MALDI-TOF |
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