汉江流域不同土地利用方式下土壤反硝化率及其控制因素研究

Land-use patterns can affect various nutrient cycles in stream ecosystems, but little information is available about the effects of urban development on denitrification processes at the watershed scale. In the presented study, we investigated the controlling factors of denitrification rates within the streams of the Han River Basin, Korea, with different land-use patterns, in order to enhance the effectiveness of water resource management strategies. Ten watersheds were classified into three land-use patterns （forest, agriculture and urban） using satellite images and geographic information system techniques, and in-situ denitrification rates were determined using an acetylene blocking method. Additionally, sediment samples were collected from each stream to analyze denitrifier communities and abundance using molecular approaches. In-situ denitrification rates were found to be in the order of agricultural streams （289.6 mg N20-N m-2 d-1） 〉 urban streams （157.0 mg N20-N m-2 d-1） 〉 forested streams （41.9 mg N20-N m-2 d-l）. In contrast, the average quantity of denitrifying genes was the lowest in the urban streams. Genetic diversity of denitrifying genes was not affected by watershed land-use pattern, but exhibited stream-dependent pattern. More significance factors were involved in denitrification in the sites with higher denitrification rates. Multiple linear regression analysis revealed that clay, dissolved organic carbon and water contents were the main factors controlling denitrification rate in the agricultural streams, while dissolved organic carbon was the main controlling factor in the urban streams. In contrast, temperature appeared to be the main controlling factor in the forested streams.

Denitrification rates and their controlling factors in streams of the Han River Basin with different land-use patterns

Land-use patterns can affect various nutrient cycles in stream ecosystems, but little information is available about the effects of urban development on denitrification processes at the watershed scale. In the presented study, we investigated the controlling factors of denitrification rates within the streams of the Han River Basin, Korea, with different land-use patterns, in order to enhance the effectiveness of water resource management strategies. Ten watersheds were classified into three land-use patterns (forest, agriculture and urban) using satellite images and geographic information system techniques, and in-situ denitrification rates were determined using an acetylene blocking method. Additionally, sediment samples were collected from each stream to analyze denitrifier communities and abundance using molecular approaches. In-situ denitrification rates were found to be in the order of agricultural streams (289.6 mg N₂O-N m^-2 d^-1) > urban streams (157.0 mg N₂O-N m^-2 d^-1) > forested streams (41.9 mg N₂O-N m^-2 d^-1). In contrast, the average quantity of denitrifying genes was the lowest in the urban streams. Genetic diversity of denitrifying genes was not affected by watershed land-use pattern, but exhibited stream-dependent pattern. More significance factors were involved in denitrification in the sites with higher denitrification rates. Multiple linear regression analysis revealed that clay, dissolved organic carbon and water contents were the main factors controlling denitrification rate in the agricultural streams, while dissolved organic carbon was the main controlling factor in the urban streams. In contrast, temperature appeared to be the main controlling factor in the forested streams.