玉米几丁质酶基因导入甘蓝型油菜的研究

以甘蓝型油菜（Brassica napus L.)下胚轴为转化材料，通过根癌农杆菌（Agrobacterium tumefaciens)介导将玉米几丁质酶基因（Maize Chitinase gene)导入双低油菜品种D2中，获得了抗卡那霉素（Kanamycin Kan)的再生植株，并对影响遗传转化的一些因素进行了研究。结果表明：适当的预培养对油菜下胚轴的遗传转化是必要的；油菜下胚轴遗传转化过程中的过敏反应可通过一些措施缓解；延迟筛选可提高油菜下胚轴的转化率。对抗性植株进行PCR检测表明：部分抗性植株在多次重复检测中的均显示较强的阳性反应，初步证明外源基因已整合到油菜基因组中。

INCORPORATING MAIZE CHITINASE INTO RAPESEED ( BRASSICA NAPUS) BY AGROBACTERIUM TUMEFACIENS

In an experiment with the hypocotyls of rapeseed (Brassica napus L.) as explants in in vitro culture,a transformation of introducing maize chitinase gene into the double-low rapeseed cultivar D 2 by Agrobacterium tumefaciens was established. Some Kan-resistant green shoots were obtained.It was shown that pre-culture was essential for the transformation of the hypocotyls of rapeseed,the hypersensitive responses of the cultured hypocotyls in transformation could be alleviated by some measures,and delayed selection was advantageous to increasing transformation frequency.PCR test of the resistant plants indicated that some of the Kan-resistant plantlets showed strong positive reaction in repeated tests,suggesting that the foreign gene had been integrated into the genome of rapeseed.