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| TC/IC-RT-PCR检测黄瓜绿斑驳花叶病毒 | |
| 引用本文： | 沈建国,林双庆,蔡伟,李海明,王念武,翁瑞泉,黄可辉.TC/IC-RT-PCR检测黄瓜绿斑驳花叶病毒[J].福建农林大学学报(自然科学版),2012,41(1):13-17. |
| 作者姓名： | 沈建国 林双庆 蔡伟 李海明 王念武 翁瑞泉 黄可辉 |
| 作者单位： | 1. 福建出入境检验检疫局,福建省检验检疫技术研究重点实验室,福建福州350001 2. 福建农林大学生命科学学院,福建福州,350002 3. 福建省农业科学院,福建福州,350001 |
| 基金项目： | 福建省自然科学基金项目(2009J01046);福建省科技计划重点项目(2009N0001);福建出入境检验检疫局科技项目(FK2011-07、FK2008-05、FK2006-19) |
| 摘 要： | 根据GenBank上登录的黄瓜绿斑驳花叶病毒(CGMMV)外壳蛋白基因保守序列,设计特异性引物,利用试管捕捉RT-PCR(TC-RT-PCR)和免疫捕捉RT-PCR(IC-RT-PCR)技术对黄瓜绿斑驳花叶病毒进行检测.结果表明,TC-RT-PCR和IC-RT-PCR均具有良好的特异性,检测灵敏度分别比DAS-ELISA高10和100倍;通过对一批进境黄瓜样品的检测,验证了2种检测技术的实际应用效果.因此,本研究建立的TC-RT-PCR和IC-RT-PCR检测技术,可有效应用于CGMMV的快速检测. |
| 关 键 词： | 黄瓜绿斑驳花叶病毒 试管捕捉RT-PCR 免疫捕捉RT-PCR |
Detection of cucumber green mottle mosaic virus by TC/IC-RT-PCR |
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| SHEN Jian-guo , LIN Shuang-qing , CAI Wei , LI Hai-ming , WANG Nian-wu , WENG Rui-quan , HUANG Ke-hui.Detection of cucumber green mottle mosaic virus by TC/IC-RT-PCR[J].Journal of Fujian Agricultural and Forestry University,2012,41(1):13-17. | |
| Authors: | SHEN Jian-guo LIN Shuang-qing CAI Wei LI Hai-ming WANG Nian-wu WENG Rui-quan HUANG Ke-hui |
| Institution: | 1(1.Fujian Exit-Entry Inspection and Quarantine Bureau,Fujian Key Laboratory for Technology Research of Inspection and Quarantine,Fuzhou,Fujian 350001,China;2.College of Life Sciences,Fujian Agriculture and Forestry University, Fuzhou,Fujian 350002,China;3.Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350001,China) |
| Abstract: | Specific primers were designed based on the conserved region of coat protein(CP) gene of Cucumber green mottle mosaic virus(CGMMV).Tube capture RT-PCR(TC-RT-PCR) and immunocapture RT-PCR(IC-RT-PCR) were employed to detect CGMMV.The results showed that two assay technologies had high specificity.Sensitivity provided by TC-RT-PCR and IC-RT-PCR was 10 and 100 times higher than that of DAS-ELISA,respectively.The application effects of two technologies were confirmed by means of detecting CGMMV in imported cucumber samples.TC-RT-PCR and IC-RT-PCR developed in this study can be effectively used for the rapid detection of CGMMV. |
| Keywords: | Cucumber green mottle mosaic virus tube capture RT-PCR immunocapture RT-PCR |
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