| 基因组荧光原位杂交区分百合回交一代的不同基因组* |
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| 引用本文： | 周树军.基因组荧光原位杂交区分百合回交一代的不同基因组*[J].园艺学报,2003,30(4):485-486. |
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| 作者姓名： | 周树军 |
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| 作者单位： | (山东农业大学林学院，泰安271018) |
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| 摘 要： | 利用东方百合(Oriental Lily)基因组DNA作荧光原位杂交的探针，用鲱鱼精子DNA或亚洲百合(Asiatic Lily)基因组DNA作封阻DNA，对东方百合和亚洲百合杂种的回交一代(Bc )的中期染色体进行了荧光染色。该方法将东方百合和亚洲百合的两个基因组在其回交一代中清楚地区分开来。表明基因组荧光原位杂交技术既能有效地鉴定杂交的成败，又能科学地分析基因组之间染色体的交换和重组。关键词：中图分类号：
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| 关 键 词： | 百合 亚洲百合 东方百合 基因组荧光原位杂交 核型 |
| 文章编号： | 0513-353X(2003)04-0485-02 |
Discrimination of the Genomes in BC1 Progeny of Asiatic Lily and Oriental Lily Using GISH |
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| Zhou Shujun.Discrimination of the Genomes in BC1 Progeny of Asiatic Lily and Oriental Lily Using GISH[J].Acta Horticulturae Sinica,2003,30(4):485-486. |
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| Authors: | Zhou Shujun |
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| Institution: | (College ofForesty，Shandong Agricultural University，Ta’an 271018，China) |
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| Abstract: | Genomic in situ hybridization(GISH)was used to discriminate the different genomes in the BC 1 progeny of Oriental lily and Asiatic lily.We used genomic DNA of Oriental Lily as probe and HS sperm DNA or genomic DNA of Asiatic Lily as block DNA for chromosome painting in the present paper.The result clearly showed the difference between the two genomes of Oriental Lily and Asiatic Lily in their BC 1.This confirmed that GISH could be used not only to identify the successfulness of the cross between Oriental Lily and Asiatic Lily but also to analyze the chromosome crossover or recombination between the two different genomes. |
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| Keywords: | Asiatic Lily Oriental Lily Genomic in situ hybridization(GISH) Karyotype |
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