| 杜仲组织培养的研究 |
| |
| 引用本文： | 何泼,申延,秦俊哲,董文宾. 杜仲组织培养的研究[J]. 林业科技开发, 2006, 20(3): 27-30 |
| |
| 作者姓名： | 何泼 申延 秦俊哲 董文宾 |
| |
| 作者单位： | 陕西科技大学生命科学与工程学院 |
| |
| 基金项目： | 陕西科技大学博士基金(编号:BJ04-03)。 |
| |
| 摘 要： | 以杜仲无菌苗的幼茎为外植体接种于MS培养基上,并附加相应的植物生长调节剂进行杜仲愈伤组织的诱导和增殖的研究;另外,对不同培养基、光照条件、pH值等理化因子对杜仲愈伤组织增殖的影响进行了研究。结果表明,MS培养基(0．8％琼脂 3％蔗糖)附加2．0 mg／L NAA 0．5 mg／L6-BA有利于杜仲愈伤组织的诱导。B5培养基(0．8％琼脂 3％蔗糖)附加1．0mg／LNAA 1．5mg／L6-BA,并控制pH值为6．5,12 h／d光照有利于愈伤组织的增殖。
|
| 关 键 词： | 杜仲 愈伤组织 诱导 增殖 |
| 收稿时间： | 2006-01-08 |
| 修稿时间： | 2006-01-10 |
A Study on tissue culture of Eucommia ulmoidus |
| |
| HE Po,SHEN Yan,QIN Jun-zhe,DONG Wen-bin. A Study on tissue culture of Eucommia ulmoidus[J]. China Forestry Science and Technology, 2006, 20(3): 27-30 |
| |
| Authors: | HE Po SHEN Yan QIN Jun-zhe DONG Wen-bin |
| |
| Abstract: | With the young shoots of Eucommia vlmoidues axenic as explants, the effects of basic media, types of hormone and their proportions, illumination time and pH on callus induction and proliferation of E. vlmoidues were studied in this paper. The results showed that MS medium (0.8% agar 3% sucrose) supplementing with 2.0mg/L NAA 0.5mg/L 6-BA was most suitable for the induction of Eucommia ulmoidues callus, and the B5 medium (0.8% agar 3% sucrose) supplementing with 1.0 mg/L NAA 1.5mg/L 6-BA under the condition of pH 6.5 and 12h/d illumination was optimized for the proliferation of callus. |
| |
| Keywords: | Eucommia ulmoidues Callus Induction Proliferation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
