玉米百粒重全基因组关联分析

以100份玉米自交系为材料，通过对2019-2020年山西榆次和太谷两地百粒重数据的测定，计算最佳线性无偏差预测值，结合均匀分布于玉米基因组的44 935个SNP标记，基于GLM模型和FarmCPU模型对百粒重进行全基因组关联分析(Genome-wide association study,GWAS)。关联分析结果表明，共鉴定到25个与百粒重显著关联的SNP(P<2.324e^-5)，其中，关联位点1＿274302441、1＿298518163和9＿32699254位于已定位粒重QTL(Quantitative trait loci)的bin内。在显著SNP位点的连锁不平衡范围内共挖掘出28个候选基因，其中，GRMZM2G116640(乙酰氨基葡萄糖转移酶)、GRMZM2G048733(ABA受体蛋白)、GRMZM2G106424(14-3-3类蛋白)和GRMZM2G024104(谷氨酰胺合成酶)等是重要的百粒重候选基因。

Genome-Wide Association Study of 100-kernel Weight in Maize

In this study, 100 maize inbred lines were used as materials, and the best linear unbiased predicted value was calculated by measuring the 100-kernel weight data of Yuci and Taigu in Shanxi from 2019 to 2020. Genome-wide association study(GWAS) based on GLM model and FarmCPU model for 100-kernel weight. The results of association analysis showed that a total of 25 SNPs(P<2.324e^-5) significantly associated with 100-grain weight were identified, among which the association loci 1_274302441, 1_298518163 and 9_32699254 were located in the bin of the mapped grain weight QTL(Quantitative trait loci). A total of 28 candidate genes were mined within the linkage disequilibrium of significant SNP sites, among which GRMZM2G116640(acetylglucosamine transferase), GRMZM2G048733(ABA receptor protein), GRMZM2G106424(14-3-3 class protein) and GRMZM2G024104(Glutamine synthetase) are important 100-kernel weight candidate genes.