毛桃开花相关基因PpAP2的克隆及其进化分析

为了探索毛桃开花的控制机制，开展了ap2基因的克隆研究。以毛桃品种‘冬丰’花芽为材料，应用电子克隆和PCR、RACE技术克隆ap2的cDNA全长序列，采用生物信息学方法分析基因的功能。PpAP2长度为1767 bp，开放性阅读框编码467个氨基酸残基，推断其相对分子量为52.0 kD，等电点为8.73，含有2个AP2结构域；位于毛桃基因组的scaffold_6上，而名为AP2M的SSR标记位于PpAP2内部。氨基酸同源性分析表明，PpAP2与已报道的其他植物的AP2具有较高的相似性，其中与苹果AP2同源性最高。该类基因的进化与物种进化同步。

Cloning and Phylogeny Analysis of PpAP2 Floral Homologous Genes in Peach

In order to investigate the control mechanism of peach flowering, the ap2 gene of peach was cloned. The peach (Prunus persica) cultivar‘Dongfeng’was used as material. In silico cloning PCR and RACE were employed to cloning complete cDNA sequence. The function was analyzed by bioinformatics methods. The gene had 1767 bp nucleotides and an ORF frame with 467 amino acid residue. The deduced protein had a molecular weight 52.0 kD, an equipotential point 8.73 and two copies of AP2 regions. PpAP2 was located in scaffold_6 in peach genome, and a SSR marker, namely AP2M anchored in it. Amino acid sequences alignment revealed that high similarity presented between PpAP2 and AP2 from other plant species, in which the highest homology found between PpAP2 and Malus×domestica AHAP2. The coincidence between AP2 genes revolution and species revolution was concluded.