| 菌丝生长速率法筛选纤维素降解菌的研究 |
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| 引用本文： | 王静 叶敏 范黎明 刘林 蒙远涛 但朝辉 唐志花 查友贵. 菌丝生长速率法筛选纤维素降解菌的研究[J]. 中国农学通报, 2013, 29(33): 323-326. DOI: 10.11924/j.issn.1000-6850.2012-3455 |
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| 作者姓名： | 王静 叶敏 范黎明 刘林 蒙远涛 但朝辉 唐志花 查友贵 |
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| 作者单位： | 1. 云南农业大学2. 农业生物多样性与病虫害控制教育部重点实验室/农业生物多样性应用技术国家工程研究中心/云南农业大学植物保护学院3. 云南农业大学植物保护学院农业生物多样性与病虫害控制教育部重点实验室4. |
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| 基金项目： | 国家自然科学基金项目（30960226） |
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| 摘 要： | 为了提供一种快速筛选高效纤维素降解菌的方法，并应用此方法筛选纤维素降解菌，为纤维素资源的综合开发利用奠定基础。提出了一种高效纤维素降解菌快速筛选的方法——菌丝生长速率法；从土壤中分离纯化得到31个纤维素降解菌菌株，并应用菌丝生长速率法，从中初步筛选出4个纤维素降解活性较强的菌株；进一步复筛结果表明，这4个菌株在培养12 h、24 h、36 h、48 h都表现出了较强的纤维素降解活性，且活性稳定，尤其是1号菌株，12 h纤维素降解速率达到(2.62±0.06) cm，24 h纤维素降解速率达到(5.93±0.04) cm，36 h纤维素降解速率达到(7.93±0.04) cm，48 h菌丝已经长满整个培养皿，远远高于其他菌株。
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| 关 键 词： | 紫外分光光度法 紫外分光光度法 |
| 收稿时间： | 2012-10-23 |
| 修稿时间： | 2012-11-23 |
Screening of Cellulose Degrading Fungi by Mycelium Growth Rate Method |
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| Wang Jing,Ye Min,Fan Liming,Liu Lin,Meng Yuantao,Dan Chaohui,Tang Zhihua,Zha Yougui. Screening of Cellulose Degrading Fungi by Mycelium Growth Rate Method[J]. Chinese Agricultural Science Bulletin, 2013, 29(33): 323-326. DOI: 10.11924/j.issn.1000-6850.2012-3455 |
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| Authors: | Wang Jing Ye Min Fan Liming Liu Lin Meng Yuantao Dan Chaohui Tang Zhihua Zha Yougui |
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| Affiliation: | (Key Lab of the Ministry of Education for Agro-biodiversity and Disease Control/ National Engineering Research Center Agro-biodiversity Applied Technology/ College of Plant Protection, Yunnan Agricultural University, Kunming 650201) |
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| Abstract: | In order to develop a rapid screening method for high effective cellulose degrading fungi, and to obtain the high effective cellulose degrading fungi, screening of cellulose degrading fungi by mycelium growth rate method was studied, so as to lay the foundation for the research and development of cellulose resource. Mycelium growth rate method, a new screening method of cellulose degrading fungi was offered, and by this means 31 cellulose degrading fungi were separated from the soil, and 4 fungi with strong cellulose degradation activities were got by preliminary screening and the results of the secondary screening showed that the 4 fungi had strong and stable cellulose degradation activities, and the cellulose degradation rates of strain 1 reached (2.62±0.06) cm been cultured for 12 h, (5.93±0.04) cm for 24 h, (7.93±0.04) cm for 36 h, and the mycelium covered the entire petri dish for 48 h in particular, and significantly higher than the others. |
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| Keywords: | screening |
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