Affiliation: | 1. Laboratoire de Virologie et de Biotechnologies Végétales, Institut de l'Environnement et de Recherches Agricoles (INERA), 01 BP 476 Ouagadougou 01, Burkina Faso UMR PVBMT, Centre de coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), F-97410 St Pierre, La Réunion, France UMR PVBMT, Université de La Réunion, 97410 Saint-Pierre, La Réunion, France Université Joseph Ki-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso Laboratoire Mixte International Patho-Bios, IRD-INERA, 01 BP 476 Ouagadougou 01, Burkina Faso;2. Laboratoire de Virologie et de Biotechnologies Végétales, Institut de l'Environnement et de Recherches Agricoles (INERA), 01 BP 476 Ouagadougou 01, Burkina Faso;3. UMR PVBMT, Centre de coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), F-97410 St Pierre, La Réunion, France;4. UMR PVBMT, Centre de coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), F-97410 St Pierre, La Réunion, France UMR PVBMT, Université de La Réunion, 97410 Saint-Pierre, La Réunion, France;5. Laboratoire de Virologie et de Biotechnologies Végétales, Institut de l'Environnement et de Recherches Agricoles (INERA), 01 BP 476 Ouagadougou 01, Burkina Faso Laboratoire Mixte International Patho-Bios, IRD-INERA, 01 BP 476 Ouagadougou 01, Burkina Faso;6. Université Joseph Ki-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso;7. Laboratoire de Virologie et de Biotechnologies Végétales, Institut de l'Environnement et de Recherches Agricoles (INERA), 01 BP 476 Ouagadougou 01, Burkina Faso Laboratoire Mixte International Patho-Bios, IRD-INERA, 01 BP 476 Ouagadougou 01, Burkina Faso Laboratoire National de Biosécurité, 06 BP 10798 Ouagadougou 06, Burkina Faso |
Abstract: | In West and Central Africa, as in many regions of the world, vegetables are severely affected by geminivirus diseases. In Burkina Faso, observation of various virus-like symptoms, especially on tomato, suggests the involvement of several geminiviruses and underlines the pressing need for additional information on their diversity, distribution, prevalence and host plant reservoirs. Large-scale surveys conducted in Burkina Faso confirmed the presence of tomato (yellow) leaf curl diseases (ToLCD-TYLCD) and geminiviruses in all localities with mean prevalences of 25% and 45%, respectively. Five geminiviruses including four begomoviruses (pepper yellow vein Mali virus (PepYVMLV), tomato leaf curl Burkina Faso virus, tomato leaf curl Mali virus and tomato leaf curl Ghana virus) and a dicot-infecting mastrevirus (chickpea chlorotic dwarf virus) were characterized on tomato. In addition, PepYVMLV and cotton leaf curl Gezira virus (CLCuGeV) were characterized on pepper and okra, respectively, in combination or not with alphasatellites and betasatellites for CLCuGeV. The most severe, prevalent and widely distributed virus on vegetables was PepYVMLV, which was characterized for the first time in combination with a genetically divergent DNA-B component that may constitute a key factor of PepYVMLV pathogenicity. Of the eight weeds identified as potential reservoir hosts of begomoviruses, four host PepYVMLV. The results confirm the importance of geminivirus diseases on vegetable crops in Burkina Faso and highlight the complex association of geminiviruses and satellites. The detection of begomoviruses in weeds growing close to crops points to the increasing necessity to consider reservoir plants and virus communities in the control of virus diseases. |