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Marker-assisted introgression of QTLs for yield under moisture stress into elite varieties of rice (Oryza sativa) |
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| Authors: | Jeevula N Bhukya Swarajyalakshmi N Bollineni Gopalakrishna Kadambari Reddiyamini Bommisetty Eswar R Gudikati Withanawasam M Darsha Keerthi Issa Srividhya Akkareddy Suresh N Eslavath Ajay K Dokuparthi Aparna Eragam Vinodkumar N Moode Rameshbabu Pottepalem Srilakshmi Chintala Eswarareddy P Narrareddy Bahbhen Tanti Mandal P Nimai Pallavi Muniraju Yeswanth V Janaki Lakshminarayana R Vemireddy |
| Institution: | 1. Department of Genetics and Plant Breeding, S.V. Agricultural College, Acharya NG Ranga Agricultural University (ANGRAU), Tirupati, Andhra Pradesh, India
Regional Agricultural Research Station (RARS), ANGRAU, Tirupati, India;2. Regional Agricultural Research Station (RARS), ANGRAU, Tirupati, India;3. Department of Genetics and Plant Breeding, S.V. Agricultural College, Acharya NG Ranga Agricultural University (ANGRAU), Tirupati, Andhra Pradesh, India;4. Department of Genetics and Plant Breeding, S.V. Agricultural College, Acharya NG Ranga Agricultural University (ANGRAU), Tirupati, Andhra Pradesh, India Agricultural Research Station (ARS), ANGRAU, Nellore, India;5. Agricultural Research Station (ARS), ANGRAU, Nellore, India;6. Department of Botany, Gauhati University, Guwahati, Assam, India;7. Central Rainfed Upland Rice Research Station (ICAR-National Rice Research Institute), Hazaribag, Jharkhand, India |
| Abstract: | Rice production is severely constrained by the moisture stress. The present study was undertaken to transfer two important QTLs viz., (qDTY2.2 and qDTY4.1), which controls yield under moisture stress (DTY) into two elite varieties, that is, MTU1010 and NLR34449 through marker-assisted breeding. Foreground and background selections of backcross generations, that is, BC1F1, BC2F1 and BC2F2 identified several promising introgression lines (ILs) with two QTLs and single QTLs with an appreciable level of recovery of recipient parent genome. In ILs, the recovery of MTU1010 genome content was estimated to be 83%–93% while the recovery of NLR34449 was 84%–92%. The two-QTL ILs of MTU1010 and NLR34449 backgrounds (qDTY2.2 and qDTY4.1) have shown substantial yield advantage (32 to 84%) over the single-QTL ILs (either qDTY2.2 or qDTY4.1) under moisture stress conditions. Among all, two ILs, MBC-124 and NBC-127 are the best high yielding lines under moisture stress conditions. These outyielded ILs have the potential to be released as varieties in rainfed ecosystem and also can be used as donors in the existing breeding programme in rice. |
| Keywords: | drought tolerance introgression marker-assisted breeding moisture stress qDTY QTLs rice |