| 贵州修文猕猴桃溃疡病菌遗传多样性分析 |
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| 引用本文： | 黄露,吴石平,谭清群. 贵州修文猕猴桃溃疡病菌遗传多样性分析[J]. 中国果业信息, 2021, 50(1) |
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| 作者姓名： | 黄露 吴石平 谭清群 |
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| 作者单位： | 贵州省植物保护研究所,贵州省植物保护研究所,贵州省植物保护研究所 |
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| 基金项目： | 贵阳市与省农科院农业科技合作项目“猕猴桃溃疡病和根结线虫病综合防治技术研究与示范”(院地农科合字[2014]4号)；贵州省科研机构服务企业行动计划项目[黔科合服企(2015)4012]；贵州省科技支撑项目“猕猴桃软腐病、溃疡病、蚧壳虫绿色防控技术研究与示范-2” [[黔科合支撑(2017)2566-2]；贵州省科技计划项目“基于转录组学对溃疡病菌与猕猴桃的互作机制解析” [黔科合基础(2019)1308]。 |
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| 摘 要： | 采用16S rDNA序列分析及ERIC-PCR对从贵州修文贵长猕猴桃枝条、叶片、花蕾分离到的35株猕猴桃溃疡病菌进行遗传多样性分析。通过16S rDNA序列分析,鉴定所分离的病原菌为Pseudomonas syringae pv.actinidiae,并运用ERIC-PCR分析病菌群体的遗传多样性分析,相似系数为0.808时,35株菌株分为4个类群,85.7%的菌株属于第一个类群,且菌株无明显的采集地、采集部位的聚类；表明修文猕猴桃溃疡病菌具有丰富的遗传多样性；此研究为溃疡病的检测、防治提供科学依据。
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| 关 键 词： | 猕猴桃 猕猴桃溃疡病菌 遗传多样性 ERIC-PCR |
| 收稿时间： | 2020-03-02 |
| 修稿时间： | 2020-12-11 |
Genotypic Diversity of Pseudomonas syringae pv. actinidiae isolates from kiwifruit on Xiuwen of Guizhou province |
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| Abstract: | The analysis of genotypic diversity of 35 isolates of Pseudomonas syringae pv.actinidiae isolated from branches,leaves and flowers of Guichang kiwifruit from Xiuwen,Guizhou by 16S rDNA sequence and ERIC-PCR. The isolates were identified as Pseudomonas syringae pv.actinidiae by 16S rDNA sequence analysis.The genetic diversity analysis of the strains using ERIC-PCR showed that 35 isolates were clustered into 4 branches at the threshold of genetic similar coefficient 0.808, the first cluster was the largest and included 85.7% isolates which were collected from different areas and different plant parts. The result showed that there was abundant genotypic diversity of Pseudomonas syringae pv.actinidiae from Xiuwen of Guizhou province. |
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| Keywords: | kiwifruit Pseudomonas syringae pv.actinidiae genetic diversity ERIC-PCR |
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