Protective effect of vitamin C and lycopene on the in vitro fertilization capacity of sex-sorted bull sperm by inhibiting the oxidative stress |
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Authors: | Pei-Pei Zhang Jing-Jing Wang Chong-Yang Li Hai-sheng Hao Hao-Yu Wang Wei-Hua Du Yun-Wei Pang Shan-Jiang Zhao Hui-Ying Zou Tong Hao Sha Yang Wei-Min Ruan Jin-Ming Huang Peng-cheng Jiao Hua-Bin Zhu Xue-Ming Zhao |
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Affiliation: | 1. Embryo Biotechnology and Reproduction Laboratory and the Center of Domestic Animal Reproduction & Breeding, Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China;2. International Joint Center for Biomedical Innovation, School of Life Sciences, Henan University, Kaifeng, China;3. Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, Jinan, China;4. Center of Biomedical Analysis, Tsinghua University, Beijing, China |
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Abstract: | The fertilization capacity of sex-sorted sperms is seriously decreased, which inhibits its wide application. However, little information is still available about the effect of vitamin C (VC) and lycopene (Lyc) on the fertilization capacity of sex-sorted bull sperm. In this study, the washing medium and fertilization medium of sex-sorted sperm from three bull individuals were supplemented with different concentrations of VC (0, 1 × 10–3, 1 × 10–4, 1 × 10–5, 1 × 10–6 M) or Lyc (0, 1 × 10–4, 1 × 10–5, 1 × 10–6, 1 × 10–7). After washing twice and incubation for 1.5 hr, the malondialdehyde (MDA) level, phosphatidylserine (PS) translocation, membrane potential (Δψm) and IVF (in vitro fertilization) ability of sex-sorted sperm were investigated. For the sex-sorted sperm of bulls A, B and C, 1 × 10–3 M VC or 1 × 10–4 M Lyc treatment significantly decreased their MDA levels and PS translocation and increased their Δψm levels and cleavage rates after IVF. When blastocysts were concerned, 1 × 10–4 M Lyc significantly improved the blastocyst rates and their IFN-tau expression of bulls A and C. In conclusion, supplementation of 1 × 10–3 M VC or 1 × 10–4 M Lyc in washing and fertilization medium contributed greatly to improving the fertilization capacity of sex-sorted bull sperm during IVF procedure. |
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Keywords: | in vitro fertilization oxidative stress sexual selection sperm |
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