Ovaprim treatment promotes oocyte development and milt fertilization rate in diploid and triploid African catfish (<Emphasis Type="Italic">Clarias gariepinus)</Emphasis> |
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Authors: | Ali Karami Annie Christianus Hadi Zokaeifar Khairul Zamri Saad Fahmi T J Imraan Shahram Shakibazadeh Hossien Negarestan Simon C Courtenay |
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Institution: | (1) Department of Aquaculture, Faculty of Agriculture, University Putra Malaysia, 43400 Selangor, Malaysia;(2) Department of Aquatic Ecology of Caspian Sea, Iranian Fisheries Research Organization (IFRO), Tehran, Iran;(3) Fisheries and Oceans Canada at the Canadian Rivers Institute, Department of Biology, University of New Brunswick, Fredericton, NB, Canada |
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Abstract: | Triploid fish are increasingly used in aquaculture because they are generally unable to reproduce successfully. Energy is
channeled into somatic growth rather than gonadal development, and in the event of escape, the animals are unlikely to breed
successfully among themselves or with wild conspecifics. This study tested the ability of recently matured triploid African
catfish (Clarias gariepinus) to produce and fertilize eggs with and without ovaprim treatment. Triploid females did not show the increase in ovary size
observed in diploid members of the same cohort between 8 and 9 months of age, or the coincident decrease in visceral fat deposits,
and this was unaffected by up to 5 weekly i.m. injections of 0.5 ml kg−1 Ovaprim. However, we observed advanced vitellogenin (Vtg) sequestration in oocytes of triploid females, albeit to a lesser
degree and with lesser cortical alveoli, compared to oocytes from diploid cohort members. Histological sections revealed a
positive trend of oocyte development up to the third weekly ovaprim injection followed by a negative gonadal development in
weeks four and five. Milt from triploid males injected 9–12 h earlier with 0.25 ml kg−1 ovaprim i.m. fertilized more diploid eggs than milt from untreated triploid males (30 vs. 20%), but none of the developing
embryos of triploid paternity survived to hatch. In contrast, milt of diploid males fertilized 49% of eggs, and 20% of the
developing embryos hatched successfully. These rates were improved in ovaprim-injected diploid males to 70% fertilization
and 33% hatch. This study demonstrates potential of overcoming non-viability of eggs from triploid female African catfish,
and enhancing the ability of triploid milt to fertilize eggs. |
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