| 新城疫病毒单克隆抗体的制备及ELISA检测方法的建立 |
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| 引用本文： | 赵坤,李任峰,赵朴,赵恒章,常新耀.新城疫病毒单克隆抗体的制备及ELISA检测方法的建立[J].河南农业大学学报,2011,45(4):428-431. |
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| 作者姓名： | 赵坤 李任峰 赵朴 赵恒章 常新耀 |
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| 作者单位： | 河南科技学院动物科学学院,河南新乡,453003 |
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| 基金项目： | 河南省基础与前沿技术研究计划项目(072300430020) |
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| 摘 要： | 将复性的重组NDV HN蛋白免疫BALB/c小鼠的脾细胞与SP2/0骨髓瘤细胞融合制备杂交瘤.通过ELISA筛选,获得了1株稳定分泌抗HN单克隆抗体(MAb)的杂交瘤细胞4E11.ELISA和Western-blot结果表明,该MAb能与NDV特异性反应,而不与H9亚型AIV,IBDV,EDS76,IBV和CIAV交叉...
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| 关 键 词： | 新城疫病毒 单克隆抗体 ELISA |
Development of monoclonal antibody against NDV and establishment of ELISA detection method |
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| ZHAO Kun,LI Ren-feng,ZHAO Pu,ZHAO Heng-zhang,CHANG Xin-yao.Development of monoclonal antibody against NDV and establishment of ELISA detection method[J].Journal of Henan Agricultural University,2011,45(4):428-431. |
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| Authors: | ZHAO Kun LI Ren-feng ZHAO Pu ZHAO Heng-zhang CHANG Xin-yao |
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| Institution: | ZHAO Kun,LI Ren-feng,ZHAO Pu,ZHAO Heng-zhang,CHANG Xin-yao(Department of Animal Science and Technology,Henan Institute of Science andTechnology,Xinxiang 453003,China) |
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| Abstract: | BALB/c mice were immunized with the recombinant HN protein of NDV.Hybridomas was generated by the fusion of the spleenocytes from these mice with SP2/0 myeloma cells.Hybridoma cells were screened by ELISA.One hybridoma cell line(4E11) stable in secreting anti-HN monoclonal antibodies(MAb) was generated.Specificity of the MAbs was identified by ELISA and Western blot.The results showed that the MAb can specifically react with NDV without cross-reactivity to H9-AIV,IBDV,EDS76,IBV and CIAV.Double antibody sand... |
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| Keywords: | NDV monoclonal antibodies ELISA |
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