利用菊芋一步法生产酒精工程菌构建的研究

为了构建一种能够直接利用菊芋（Helianthus tuberosus）来进行酒精发酵的融合菌株，选择适宜的原生质体制备与融合条件，将自行筛选所得的一种能高产菊粉酶的青霉菌与酿酒酵母进行原生质体融合，选择性地检出融合子，对其产酶能力、产酒精能力进行测定，并对其遗传稳定性进行研究，最终确定筛选出一株各项性能较优的融合菌株R8。酒精发酵试验结果显示，菌株R8发酵菊粉的酒精得率为41.16%，原料糖分利用率为75.52%。

Construction of engineered bacteria for onestep producing alcohol with Jerusalem artichoke

To obtain fusant strains being able to produce ethanol directly from Jerusalem artichoke(Helianthus tuberosus) materials efficiently,the protoplast fusion technique was used and some appropriate conditions were chosen for protoplast preparation and fusion.The protoplast fusion was conducted between Saccharomyces cerevisiae and a penicillium strain obtained by self-screening with a high yield of inulinase.The abilities of inulinase and ethanol production of detected fusants was determined.Their genetic stability was also studied.Finally,a superior strain named R8 was chosen for inulin alcoholic fermentation.The results showed that the ethanol content of fermented products was 41.16% and the raw sugar utilization rate was 75.52%.