Gateway技术构建结缕草低温和干旱诱导cDNA文库及其质量鉴定

为研究结缕草（Zoysia japonica）胁迫响应信号转导途径中的关键基因，揭示结缕草抗逆分子机制，建立结缕草功能基因组学研究基础平台，以经低温、干旱处理的结缕草为材料，采用Gateway技术构建了首个结缕草低温和干旱诱导的标准cDNA文库。文库质量分析表明，未扩增的原始文库滴度1.76×106 pfu·mL-1，库容7.04×106 pfu，插入片段平均大小大于1 kb，重组率90%。文库质量优良，可能包含大量新基因，不仅能为结缕草功能基因组分析提供必要资源，也可为后期进行高通量EST测序、发掘新抗逆相关基因、制作基因芯片等研究奠定基础。

Construction and identification of a cold-and drought-induced cDNA library from zoysiagrass using Gateway technology

The first cDNA library was constructed using Gateway technology from zoysiagrass(Zoysia japonica) subjected to cold-and drought-stress treatments.Quality assessment showed that the titer of unamplified library was 1.76×106 pfu·mL-1 and the capacity was 7.04×106 pfu.The average insert size was larger than 1 kb with the recombination efficiency of 90%.It was suggested that the cDNA library was successfully generated in high quality;most likely containing lots of novel genes.The library may provide an essential resource for future functional genomic analysis of zoysiagrass and could be an effective tool for further studies on high-throughput EST sequencing,for example,new stress-responsive genes screening,gene chips preparing.