长叶点地梅愈伤组织诱导和植株再生

以长叶点地梅（Androsace longifolia）的无菌实生苗的下胚轴和叶片为外植体，研究不同种类、不同浓度的激素组合培养对愈伤组织诱导、芽诱导和生根的影响。结果表明，诱导下胚轴基部愈伤组织的最佳培养基为MS+0.3 mg·L-1 6 BA+0.1 mg·L-1 NAA，诱导率达85%；诱导叶片愈伤的最佳培养基为MS+0.02 mg·L-1 NAA+0.3 mg·L-1 TDZ，诱导率为80%；下胚轴愈伤诱导芽的最佳培养基为MS+0.5 mg·L-1 6 BA+0.2 mg·L-1 NAA，分化率达92.5%；叶片愈伤诱导芽的最佳培养基为MS+1.0 mg·L-1 6 BA+0.2 mg·L-1 NAA，分化率达82.5%；最佳生根培养基为MS+1.0 mg·L-1 IBA，生根率达100%，移栽成活率达95%。

Callus induction and plant regeneration of Androsace longifolia

The hypocotyls and leaves from the sterile seedlings of Androsace longifolia were taken as explants to study the effects of different hormone compositions on callus induction,bud induction and rooting.The optimum medium for hypocotyls callus induction was MS + 0.3 mg·L-1 6-BA + 0.1 mg·L-1 NAA,and the induction rate reached 85%.The medium MS + 0.02 mg·L-1 NAA + 0.3 mg·L-1 TDZ was better for leaf callus induction,and the induction rate reached 80%.The buds were induced in medium MS + 0.5 mg·L-1 6-BA + 0.2 mg·L-1 NAA from hypocotyls callus,and the differentiation rate reached 92.5%.The medium MS + 1.0 mg·L-1 6-BA + 0.2 mg·L-1 NAA was optimal for inducing buds from leaf callus,and the differentiation rate was the highest(82.5%).The best rooting medium was medium MS + 1.0 mg·L-1 IBA.The rooting rate reached 100% and survival rate reached 95%.