油茶SOD基因片段克隆及序列分析

摘要：根据已报道的多种植物Cu/Zn SOD和Mn/Fe SOD基因的氨基酸序列的保守区域分别设计简并引物，以油茶（Camellia oleifera）叶片总RNA为模板，通过RT PCR分别扩增得到一个375 bp的Cu/Zn SOD基因片段和一个429 bp的Mn/Fe SOD基因片段。将片段序列在NCBI网站上进行同源性比对，表明本研究克隆的结果均与多种植物的Cu/Zn SOD和Mn/Fe SOD基因存在亲缘关系，因而认为所克隆的基因片段就是油茶SOD基因；并运用DNAStar5.0软件分别进行序列分析，推导的氨基酸序列分别为125个残基和143个残基；具有所有植物SOD基因共有的保守区域；与多种植物Cu/Zn SOD基因的核苷酸和氨基酸序列的同源性均分别在80%和84%以上，与其他植物的Mn/Fe SOD基因的核苷酸和氨基酸序列的同源性都在84%和86%以上。

Cloning and sequence analysis of SOD gene of Camellia oleifera

Two pairs of degenerate primers were designed based on the conservative regions in amino acids of Cu/Zn-SOD and Mn/Fe-SOD genes from other plants,respectively.Cu/Zn-SOD gene fragment and Mn/Fe-SOD gene fragment of Camellia oleifera were cloned.Their sizes were 375 bp and 429 bp,respectively.By using the program of BLAST on NCBI GenBank database,two sequences presented a very high identity with Cu/Zn-SOD and Mn/Fe-SOD genes from other plants.The nucleotide sequences were analyzed by using program of DNAStar 5.0.125 amino acids and 143 amino acids were coded by the nucleotide sequences and the nucleotide sequences had the same conservative regions of Cu/Zn-SOD and Mn/Fe-SOD genes as many kinds of other plants.The homology comparison of nucleotide sequences of Cu/Zn-SOD gene and Mn/Fe-SOD gene showed the similarities of more than 80% and 84% to other plants,respectively.The similarities of amino acid sequences of Cu/Zn-SOD and Mn/Fe-SOD genes were more than 84% and 86% to other plants.