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百合属‘普瑞头’的组织培养和快速繁殖
引用本文:张彦妮,李文英.百合属‘普瑞头’的组织培养和快速繁殖[J].草业科学,2012,29(7):1077-1083.
作者姓名:张彦妮  李文英
作者单位:东北林业大学园林学院,黑龙江哈尔滨,150040
基金项目:中央高校基本科研业务费专项资金项目
摘    要:以亚洲百合‘普瑞头’(Lilium asitic hybrids cv. Prato)鳞片为外植体,以MS为基本培养基,通过增加不同激素种类和浓度进行了组织培养快速繁殖技术的研究。结果表明,诱导不定芽的最适培养基为MS+0.5 mg·L-1 6 BA+0.1 mg·L-1 NAA,诱导率最高达85.0%,平均每块分化的芽数最多(3.6个);再生小鳞茎鳞片诱导不定芽的最适培养基为MS+1.0 mg·L-1 6 BA;生根的最适宜培养基为1/2 MS+0.5 mg·L-1 IBA,生根率为86.7%;移栽后生长良好,成活率达到85.0%。

关 键 词:‘普瑞头’鳞片  组织培养  快繁

Tissue culture and rapid propagation of Lilium asitic hybrids cv. Prato
ZHANG Yan-ni,LI Wen-ying.Tissue culture and rapid propagation of Lilium asitic hybrids cv. Prato[J].Pratacultural Science,2012,29(7):1077-1083.
Authors:ZHANG Yan-ni  LI Wen-ying
Institution:(College of Landscape Architecture,Northeast Forestry University,Harbin 150040,China)
Abstract:The rapid propagation technique was studied in Lilium asitic hybrids cv.Prato with the bulb scales and leaves in vitro as explants and MS as basic medium with different hormones and concentrations.The results showed that the best medium for adventitious bud induction from bulb scales and leaves was MS+0.5 mg·L-1 6-BA+0.1 mg·L-1 NAA,with which the highest induction rate was 85.0%,and the average number of shoots from every explant was 3.6.The best medium for adventitious buds induced from bulb scales was MS+1.0 mg·L-1 6-BA.The optimum medium for taking root was 1/2 MS+0.5 mg·L-1 IBA,and the rooting rate was 86.7%.The transferred plants,with a survival rate of 85.0%,grew well.
Keywords:Lilium asitic hybrids cv  Prato  bulb scales  tissue culture  rapid propagation
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