20份马蹄金种质资源遗传多样性RAPD和SRAP分析

以1份美国进口马蹄金(Dichondra repens)品种为对照，用RAPD和SRAP两种分子标记方法研究20份野生马蹄金种质资源的遗传多样性。结果发现，20条RAPD引物扩增出多态性条带130条，多态性比率为68.78％；22对SRAP引物扩增出175条多态性条带，多态性比率为72.31％，表明20份野生马蹄金之间具有较丰富的遗传多样性。聚类结果表明，RAPD标记将供试材料聚为两大类群，SRAP标记也将材料划分为两大类。两种标记下，供试材料呈现出较好的地域性分布规律，地理来源相近的材料能大致聚为一类，部分同聚为一类的材料在形态上也具有相似性。两种标记方法相关系数r0.01=0.910，二者存在极显著正相关，表明应用这两种技术对马蹄金遗传多样性与亲缘关系的分析具有较高的一致性和可信度。

Genetic diversity analysis of 20 Dichondra repens germplasm by RAPD and SRAP

Genetic diversity of 20 Dichondra repens wild germplasm collected from different location in southwest China,compared with an imported American variety(Common),were analyzed by RAPD and SRAP markers.The results showed that 20 RAPD primers produced 189 bands,including 130 polymorphic bands;the average percentage of polymorphic bands was 68.78%.22 pairs of SRAP primers produced 242 bands,including 175 polymorphic bands;the average percentage of polymorphic bands was 72.31%.The 21 germplasm could be clustered into either two groups based on RAPD markers as well as SRAP markers.Moreover,the tested germplasm with same origin or similar morphology frequently clustered into the same group and showed a nice geographical distribution and relationships.There was significant positive correlation between RAPD and SRAP(r0.01=0.910),which indicated that the two methods were high consistant and reliable for detecting genetic diversity and genetic relationships in D.repens germplasms,though some germplasm were clustered into different groups between the two markers.