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牛初乳sIgA分离纯化及其检测方法的研究进展
引用本文:刘晓飞,高学军,姚永豪,李殿胜.牛初乳sIgA分离纯化及其检测方法的研究进展[J].东北农业大学学报,2008,39(10).
作者姓名:刘晓飞  高学军  姚永豪  李殿胜
作者单位:东北农业大学乳品科学教育部重点实验室,哈尔滨,150030;哈尔滨绿达生动物药业有限公司,啥尔滨,150039
摘    要:利用牛初乳开发新的功能性食品和类母乳化食品是当前研究热点,而在牛初乳中,sIgA是一种重要的免疫球蛋白,因此分离及检测牛初乳中sIgA具有重大现实意义。目前分离纯化方法主要有亲和色谱法、嗜硫色谱法、离子交换法、超滤法、酶解法等,检测方法有高效液相色谱法、酶联免疫吸附测定法、辣根过氧化物酶标记凝集素法、免疫胶体金检测法等。文章对sIgA分离纯化及检测方法进行了简要介绍。

关 键 词:牛初乳  分泌型免疫球蛋白A  分离纯化  检测

Research progress on purification and characterization of bovine colostrum secretory immunoglobulin A
LIU Xiaofei,GAO Xuejun,YAO Yonghao,LI Diansheng.Research progress on purification and characterization of bovine colostrum secretory immunoglobulin A[J].Journal of Northeast Agricultural University,2008,39(10).
Authors:LIU Xiaofei  GAO Xuejun  YAO Yonghao  LI Diansheng
Institution:LIU Xiaofei1,GAO Xuejun1,YAO Yonghao1,LI Diansheng2(1.Key Laboratory of Dairy Science,Ministry of Education,Northeast Agricultural University,Harbin 150030,China,2.Harbin Lvdasheng Veterinary Pharmaceutical Co.,Ltd.,Harbin 150039,China)
Abstract:Functional foods and humanized foods developing are currently key points of utilization of bovine colostrum,and bovine colostrum sIgA is one of the most important immunoglobulins,so the purification and the characterization of bovine colostrum sIgA have greatly practical importance.There are several methods in bovine colostrum sIgA purification,for example,affinity chromatography,thiophilic adsorption chromatography,ion ex-change chromatography,ultrafiltration and enzymolysis.The methods of characterization...
Keywords:bovine colostrums  secretory immunoglobulin A  purification  characterization  
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