传染性支气管炎病毒青岛腺胃分离株(SD/97/02)基质蛋白、5a、5b 蛋白基因的克隆和序列分析

参考 Gen Bank上的传染性支气管炎病毒 (IBV)序列 ,自行设计合成了 3条引物 ,对 IBV青岛腺胃分离株 (SD/97/ 0 2 ) RNA进行 RT- PCR扩增 ,扩增含基质蛋白 (M)及 5 a、5 b蛋白基因的约 1.6 5 kb的片段 ,对 PCR产物进行克隆后测序。序列分析显示 ,M蛋白基因与其他 IBV相应的基因同源性在 90 .33%～ 92 .75 %之间 ,氨基酸序列比较 ,同源性在 89.82 %～ 94.2 5 %之间 ;5 a基因与其他 IBV的基因同源性在 84.34 %～ 87.37%之间 ,氨基酸同源性在 81%～82 %;5 b基因与其他 IBV的基因同源性在 91%～ 92 %之间 ,氨基酸同源性在 93%左右。

Cloning and Sequence Analysis of the Matrix Protein,5a and 5b Protein Genes from Infectious Bronchitis Virus Proventriculus Strain Isolated in Qingdao,China

Three primers were designed and synthesized according to the reported IBV sequences in Genbank,and the viral RNA of IBV proventriculus strain Qingdao strain(SD/97/02) was amplified through RT-PCR.A DNA fragment of about 1.6 kb in length spans matrix protein(M),5a and 5b protein genes was amplified.Then the PCR product was cloned and sequenced.Sequence analysis suggested that the gene homology of SD/97/02 M protein compared with that of other strains of IBV was between 90.33%-92.75%,in 5a gene it was 84.34%-87.37%,in 5b 91%-92%.And the amino acid homology of M,5a and 5b of SD/97/02 with those of other strains was 89.82%-94.25%,81%-82% and 93%,respectively.