Agrobacterium tumefaciens mediated genetic transformation and regeneration of Morus alba L. |
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| Authors: | Sandhya Agarwal Kamlesh Kanwar N Saini R K Jain |
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| Institution: | a Department of Biotechnology, University of Horticulture and Forestry, Solan, HP 173 230, India;b Department of Biotechnology and Molecular Biology, Haryana Agriculture University, Hisar, India |
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| Abstract: | An efficient method was established for genetic transformation of Morus alba clone M5 using Agrobacterium tumefaciens mediated gene transfer. Cotyledon explants from in vitro grown seedlings were co-cultivated with disarmed strain LBA 4404 harbouring the binary vector pBI121 carrying chimeric β-glucuronidase (GUS) and neomycin phosphotransferase (npt II) genes. Maximum transformation frequency of 18.60% was recorded with 48 h of pre-conditioning followed by co-cultivation for the same duration. Expression and presence of transgene was confirmed by histochemical test and polymerase chain reaction. The transgenic plants were micropropagated and successfully acclimatised. |
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| Keywords: | Author Keywords: Genetic transformation Morus alba L Shoot regeneration Tissue culture |
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