| 土壤中产壳聚糖酶菌株的筛选、鉴定及抑菌活性研究 |
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| 引用本文： | 马镝,赵秀香,吴元华,张晓雯. 土壤中产壳聚糖酶菌株的筛选、鉴定及抑菌活性研究[J]. 沈阳农业大学学报, 2007, 38(6): 811-815 |
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| 作者姓名： | 马镝 赵秀香 吴元华 张晓雯 |
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| 作者单位： | 沈阳农业大学,沈阳,110161 |
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| 基金项目： | 沈阳农业大学校科研和教改项目 |
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| 摘 要： | 采用透明圈法,通过大量筛选得到9株产壳聚糖酶的野生菌株,对其中产壳聚糖酶酶活最高的菌株BS-0409的菌株形态特征和生理生化特征及16S rDNA序列进行了测定分析,并对该菌的酶解产物壳寡糖进行了抑菌试验。结果表明,该菌株呈短杆状,革兰氏染色阴性,端生鞭毛。经16SrDNA序列分析表明,BS-0409菌株为巨大芽孢杆菌,该菌的酶解产物壳寡糖对17种植物病原真菌都有较强的抑制作用,抑制率为29.6%～100.0%。
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| 关 键 词： | 芽孢杆菌 壳聚糖酶 筛选和鉴定 壳寡糖 抑菌活性 |
| 文章编号： | 1000-1700（2007）06-0811-05 |
| 收稿时间： | 2007-06-11 |
| 修稿时间： | 2007-06-11 |
Selection and Identification of Chitosanase Producing Strain and Antibiotic Characterization |
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| MA Di,ZHAO Xiu-xiang,WU Yuan-hua,ZHANG Xiao-wen. Selection and Identification of Chitosanase Producing Strain and Antibiotic Characterization[J]. Journal of Shenyang Aricultural University, 2007, 38(6): 811-815 |
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| Authors: | MA Di ZHAO Xiu-xiang WU Yuan-hua ZHANG Xiao-wen |
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| Abstract: | The method of see-through circle was used to screen strains of producing chitosanase.The strain named BS-0409 which possessed the highest chitosanase activity were obtained.The strain BS-0409 was identified as Bacillus megaterium by physiological and biochemical techniques and sequence analyse of 16S rDNA.The form of strain BS-0409 is pole,G-,fiagellum from one side.The effect of chitosanase produced on phytopathogens fungi was studied.The results showed that chito-oligosaccharide had strong inhabition on several kinds of phytopathogen. |
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| Keywords: | Bacillus chitosanase screening and optimization chito-oligosaccharide antibiotic action |
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