| 农杆菌介导谷子遗传转化的研究进展 |
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| 引用本文： | 李颜方,赵晋锋,王高鸿,杜艳伟,张 正,余爱丽. 农杆菌介导谷子遗传转化的研究进展[J]. 中国农学通报, 2016, 32(36): 66-72. DOI: 10.11924/j.issn.1000-6850.casb16030213 |
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| 作者姓名： | 李颜方 赵晋锋 王高鸿 杜艳伟 张 正 余爱丽 |
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| 作者单位： | (山西省农业科学院谷子研究所/特色杂粮种质资源发掘与育种山西省重点实验室,山西长治 046011） |
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| 基金项目： | 山西省基础研究项目“谷子萌发耐旱关键基因挖掘与功能研究”(2015011071)；山西省农业科学院科技自主创新能力提升工程项目“谷子重要农艺性状的QTL定位和遗传转化体系的建立”(2016ZZCX-09)。 |
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| 摘 要： | 通过基因工程方法可以得到产量稳定增长、营养品质更高、适应能力更强的谷子品种。为了解目前谷子遗传转化体系研究的现状，本文归纳了影响谷子组织培养、农杆菌侵染和转化再生体系的关键因素，同时总结了谷子遗传转化体系的研究现状。最后指出了谷子遗传转化的瓶颈问题，展望了农杆菌介导谷子转化技术的前景，为建立稳定高效的谷子遗传转化体系提供了有益线索。
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| 关 键 词： | 再生水灌溉 再生水灌溉 作物生长 土壤养分 土壤酶 |
| 收稿时间： | 2016-03-30 |
| 修稿时间： | 2016-05-20 |
Research Advances in Agrobacterium-mediated Transformation of Millet (Setaria italica) |
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| Li Yanfang,Zhao Jinfeng,Wang Gaohong,Du Yanwei,Zhang Zheng and Yu Aili. Research Advances in Agrobacterium-mediated Transformation of Millet (Setaria italica)[J]. Chinese Agricultural Science Bulletin, 2016, 32(36): 66-72. DOI: 10.11924/j.issn.1000-6850.casb16030213 |
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| Authors: | Li Yanfang Zhao Jinfeng Wang Gaohong Du Yanwei Zhang Zheng Yu Aili |
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| Affiliation: | (Millet Research Institute, Shanxi Academy of Agricultural Sciences/Shanxi Key Laboratory of Genetic Resources and Breeding in Minor Crops, Changzhi Shanxi 046011) |
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| Abstract: | The millet varieties with steady increase of yield, higher nutritional quality and more adaptability will be obtained through genetic engineering methods. To understand the current research situation of millet genetic transformation, this paper summarized the key factors which affected the tissue culture, Agrobacterium infection and transformation regeneration system. And the research progress of millet genetic transformation was reviewed. Finally, the bottleneck problem of millet genetic transformation was pointed out, and the future application of Agrobacterium-mediated transformation technology was discussed, which provides useful clues for the establishment of millet genetic transformation system with high efficiency and stability. |
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| Keywords: | Setaria italica Agrobacterium -mediated transformation tissue culture |
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