利用PCR仪快速提取甜菜基因组DNA

为了寻找一种快速提取甜菜基因组DNA的方法，以甜菜干种子、幼苗、种仁以及甜菜叶片干粉为原料，利用PCR仪结合碱裂解法快速提取甜菜基因组DNA，利用微量分光光度计检测取DNA的浓度，并用甜菜SSR引物对提取的DNA进行扩增。结果表明，在4 种材料中均检测到了DNA，干种子、幼苗、种仁以及叶片干粉中提取的DNA平均浓度分别为432、197、158、448 ng/μL，无论是DNA原液还是稀释到20 ng/μL的工作液，均能在SSR-PCR反应中扩增出清晰的条带。该方法提取甜菜基因组DNA简单、快速，仅需要NaOH和HCl两种药品，提取的DNA完全可以用于SSR-PCR反应，为快速鉴定甜菜品种纯度和真实性提供了技术支持。

Rapid Extraction of Genomic DNA from Sugar Beet Using PCR Instrument

In order to find a method for rapidly extracting genomic DNA from sugar beet, a PCR instrument was used in combination with an alkaline lysis method with dried seeds, seedlings and kernels of sugar beet as well as dry powder of sugar beet leaf as raw materials, the concentration of extracted DNA was measured by a micro-spectrophotometer, and amplification of extracted DNA was conducted using the SSR primers of sugar beet. The results demonstrated that DNA was detected in all materials, the mean concentration of extracted DNA from dried seeds, seedlings, kernels and leaf dry powder was 432 ng/μL, 197 ng/μL, 158 ng/μL and 448 ng/μL, respectively, and clear bands were amplified in both DNA stock solution and diluted 20 ng/μL working solution by SSR-PCR reaction system. This method is simple and rapid in extracting genomic DNA from sugar beet, and only requires two medicines NaOH and HCl. The extracted DNA can be completely used for SSR- PCR reaction, which provides technical support for the rapid identification of the purity and authenticity of sugar beet varieties.