Occurrence of ‘Candidatus Phytoplasma ziziphi’ in apple trees in China |
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| Authors: | P Liu L Zhang Y‐F Wu |
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| Institution: | 1. State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture, Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, College of Plant Protection, Northwest A&F University, , Yangling, 712100 Shaanxi, China;2. College of Forestry, Northwest A&F University, , Yangling, Shaanxi, China |
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| Abstract: | Young apple trees exhibiting symptoms of little leaf, margin involute and yellows were observed in an open‐air nursery in Yangling, Shaanxi, China. Transmission electron microscopy showed typical phytoplasma bodies in the sieve tube elements of symptomatic leaf samples. In two nested polymerase chain reaction (PCR) assays, products of expected size of 1.1 and 1.2 kb were separately generated from the total DNAs of symptomatic samples. A restriction fragment length polymorphism (RFLP) analysis of the purified 1.2 kb PCR products indicated that the disease associated with apple trees was ‘Ca. Phytoplasma ziziphi’. To our knowledge, this is the first report of ‘Ca. Phytoplasma ziziphi’ infecting apple trees in China. |
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