文昌鸡促卵泡素受体基因外显子区域SNPs分析

寻找与鸡繁殖性能相关的遗传标记，为高繁殖力的标记辅助选择提供科学依据。本研究以促卵泡素受体（FSHR）基因作为影响鸡繁殖性状的候选基因，采用PCR-SSCP技术结合测序对FSHR基因部分包括所有外显子区域进行单核苷酸多态性检测和分析。结果发现，在区域2、区域4、区域6、区域9共4个区域存在SNPs位点。exon4编码区43 bp处T→C突变，但没有导致翻译后氨基酸序列的改变，在exon2中编码区5′端—49 bp处的C→T突变；exon6 编码区3′端+12 bp处的A→G突变；exon8编码区3′端+38 bp处的 G→T突变。促卵泡素受体（FSHR）是促卵泡素（FSH）的特异性受体，这些位点的单核苷酸多态性为下一步研究探讨FSHR基因对文昌鸡繁殖性能的遗传效应奠定了基础。

Single Nucleotide Polymorphisms on Follicle Stimulating Hormone Receptor

The objectives of the present study were firstly to detect polymorphisms in the follicle stimulating hormone receptor (FSHR) gene, which provide a scientific basis for marker assisted selection for high prolificacy in Wenchang chicken. In this study, the FSHR gene as the candidate gene of chicken’s reproductive trait was analysis on its regions which encode most of the extracellular domain by PCR-SSCP. The result showed that 4 SNPs sites were found at region 2, region 4, region 6, region 9, respectively. They are C-49→T-49 mutation before the CDS’ 5-flanking terminal of the exon2, T43→C43 mutation in the CDS of the exon4, A12→G12 mutation after the CDS’ 3-flanking terminal of the exon6, G38→T38 mutation after the CDS’ 3-flanking terminal of the exon8. The fitness test indicated the gene frequency were all in Hardy-Weinberg balanced state (P>0.05). In conclusions, the polymorphisms of theses sites lay a foundation for the further research on relationship between the polymorphisms of FSHR gene and the reproductive performance of Wenchang chicken.