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| 猪链球菌2型溶菌酶释放因子MRP在重组沙门菌中的表达 | |
| 引用本文： | 郑继平,郭桂英,韦双双,黄邓高,谢俊,季静,张兆山. 猪链球菌2型溶菌酶释放因子MRP在重组沙门菌中的表达[J]. 中国兽医学报, 2008, 28(11) |
| 作者姓名： | 郑继平 郭桂英 韦双双 黄邓高 谢俊 季静 张兆山 |
| 作者单位： | 1. 海南大学,农学院热带生物资源教育部重点实验室,海南,海口,570228 2. 天津大学,农业与生物工程学院,天津,300072 3. 军事医学科学院,生物工程研究所,北京,100071 |
| 基金项目： | 教育部科学技术研究重点项目，海南省自然科学基金，海南省教育厅高校科研项目，海口市农业三项资助项目 |
| 摘 要： | 从猪链球菌2型(SS2)四川分离株分离溶菌酶释放因子基因mrp,将其克隆至T载体上,再将asd基因插入到重组质粒的抗生素抗性基因中,电击转入asd缺陷型沙门菌X4072中表达,形成依赖asd基因的质粒-宿主平衡致死系统。经酶切、PCR及蛋白电泳鉴定,证实重组菌株能够稳定表达MRP,从而为SS2的防制提供了可能候选疫苗株。 |
| 关 键 词： | 猪链球菌2型 溶菌酶释放因子MRP 沙门菌 |
Expression of Streptococcus suis serotype 2 gene mrp in attenuated Salmonella X4072 |
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| ZHENG Ji-ping,GUO Gui-ying,WEI Shuang-shuang,HUANG Deng-gao,XIE Jun,JI Jing,ZHANG Zhao-shan. Expression of Streptococcus suis serotype 2 gene mrp in attenuated Salmonella X4072[J]. Chinese Journal of Veterinary Science, 2008, 28(11) | |
| Authors: | ZHENG Ji-ping GUO Gui-ying WEI Shuang-shuang HUANG Deng-gao XIE Jun JI Jing ZHANG Zhao-shan |
| Abstract: | The muramidase-released protein gene of Streptococcus suis serotypes 2 was isolated by PCR with the Streptococcus suis serotypes 2 strain from Sichuan in China,and ligated to the pMD18-T vector.The antibiotic resistance gene ampr of pMD18-T vector was destroyed by insertion of asd gene.Then the recombinant vector was transformed in Salmonella X4072 (asd-).The results of PCR,restriction enzyme digestion and SDS-PAGE indicated MRP was expressed by Salmonella X4072. |
| Keywords: | Streptococcus suis serotypes 2 muramidase-released protein Salmonella |
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