RAPD analysis of genetic relationships among Sphaeropsis sapinea isolates |
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Authors: | Xiaoqin Wu Dabin Xiong Yu Wang |
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Institution: | (1) College of Forest Resources and Environment, Nanjing Forestry University, Nanjing, 210037, China;(2) Jiangsu Forestry Administration, Nanjing, 210036, China |
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Abstract: | Genetic relationships were studied among 23 isolates of Sphaeropsis sapinea collected from China, the United States, England, South Africa and Chile by using a random amplification of a polymorphic
DNA (RAPD) analytical method. One hundred and 35 DNA fragments were amplified with 12 random primers by a polymerase chain
reaction PCR technique and 96.3% were polymorphic. The genetic dendrogram based on RAPD analysis showed that the S. sapinea isolates could be divided into three types. Isolate CWS41 from Chile was separated genetically as the first type that was
different from other isolates and isolates F2 and J2 from China comprised the second group. The third RAPD group accommodated
other isolates including the B morphotype isolate CWS43 from the United States.
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Translated from Journal of Nanjing Forestry University, 2006, 30(1): 13–16 译自: 南京林业大学学报] |
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Keywords: | Sphaeropsis sapinea random amplification of a polymorphic DNA genetic relationship |
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