水牛感染肝片吸虫后肝脏组织的病理学变化

15 头水牛在人工感染条件下进行肝片吸虫感染, 慢性感染组水牛每天口服60 个囊蚴, 20 d 总共口服1 200 个囊蚴。急性感染组水牛1 次口服1 600 个囊蚴。慢性感染的水牛于感染后第26 周宰杀。急性感染的水牛分别于第10 , 13 , 16 和22 周宰杀。肝脏的组织病理学显示, 肝片吸虫幼虫可引起肝脏一系列炎症反应。急性感染后第10 周和13 周肝细胞变性、坏死, 肝小叶内淤血, 大量淋巴细胞浸润, 继而发展为肝索萎缩。急性感染后第22 周及慢性感染后第26 周, 汇管区内有大量结缔组织及新生的小胆管增生。实验表明慢性感染和急性感染肝片吸虫的水牛均可导致肝脏典型的病理学变化。

Study on the Dynamic Changes of Liver Histopathology in Water Buffaloes During Experimental Fasciolosis

The effect of Fasciola hepatica infection on the histopathology of liver was investigated in water buffaloes.Twelve water buffaloes in chronic infection group were dosed daily with 60 F.hepatica metacercariae over 20 days and killed at 26 weeks postinfection.The water buffaloes in acute infection group were dosed with 1 600 F.hepatica metacereariae once and killed at 10 weeks,13 weeks, 16 weeks,22 weeks after infection respectively.The results showed that a series of inflammation occured in the liver during early period of infection.The hepatocytes showed degeneration,necrotic and atrophy.Foucal aggregations of a great number of lymphocytes are found in hepatic lobules following by atrophy of hepatic cords and went through a proliferation and regeneration after 22 weeks postinfection.