| 烟粉虱中甘薯双生病毒(sweepoviruses)半巢式PCR快速检测技术的建立与应用 |
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| 引用本文： | 秦艳红,王 爽,田雨婷,乔 奇,张德胜,王永江,张振臣.烟粉虱中甘薯双生病毒(sweepoviruses)半巢式PCR快速检测技术的建立与应用[J].植物保护,2022,48(1):168-172. |
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| 作者姓名： | 秦艳红 王 爽 田雨婷 乔 奇 张德胜 王永江 张振臣 |
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| 作者单位： | 河南省农业科学院植物保护研究所, 河南省农作物病虫害防治重点实验室, 农业农村部华北南部作物有害生物综合治理重点实验室, 郑州 450002 |
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| 基金项目： | 国家甘薯产业技术体系(CARS-10-B13) |
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| 摘 要： | 甘薯双生病毒(sweepoviruses)是侵染甘薯的一类重要病毒,通过烟粉虱以持久方式传播,我国甘薯上至少存在8种甘薯双生病毒.本研究根据我国已报道的8种甘薯双生病毒基因组保守区设计了一组引物,建立了单头烟粉虱中甘薯双生病毒的半巢式PCR快速检测方法.特异性和灵敏性分析结果表明,半巢式PCR具有较高的特异性和灵敏性,...
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| 关 键 词： | 甘薯双生病毒 半巢式PCR 检测 |
| 收稿时间： | 2020/10/9 0:00:00 |
| 修稿时间： | 2020/12/25 0:00:00 |
Development of semi nested PCR method for rapid detection of sweepoviruses in Bemisia tabaci |
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| QIN Yanhong,WANG Shuang,TIAN Yuting,QIAO Qi,ZHANG Desheng,WANG Yongjiang,ZHANG Zhenchen.Development of semi nested PCR method for rapid detection of sweepoviruses in Bemisia tabaci[J].Plant Protection,2022,48(1):168-172. |
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| Authors: | QIN Yanhong WANG Shuang TIAN Yuting QIAO Qi ZHANG Desheng WANG Yongjiang ZHANG Zhenchen |
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| Institution: | Institute of Plant Protection, Henan Academy of Agricultural Sciences, Henan Key Laboratory of Crop Pest Control, IPM Key Laboratory in Southern Part of North China for Ministry of Agricultural and Rural Affairs, Zhengzhou 450002, China |
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| Abstract: | Sweepoviruses are the viruses infecting sweet potato and transmitted by Bemisia tabaci in persistent manner. At least eight viruses had been identified in Chinese sweet potato. Primers were designed based on the conserved regions of genome of eight sweepoviruses from China. The rapid semi nested PCR detection technology was established for the detection of sweepoviruses in single whitefly. The semi nested PCR had high specificity and sensitivity. With the recombination plasmids of eight sweepoviruses as template, eight viruses could be simultaneously detected. The sensitivity of the semi nested PCR was higher than that of conventional PCR by 10-10 000 times. Sweepoviruses could be steadily detected from individual whitefly by using the semi nested PCR assay. This assay is of economical, rapid, sensitive, specific and stable for rapid detection of sweepoviruses in B. tabaci . |
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| Keywords: | sweepoviruses semi nested PCR detection |
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