福建省部分马铃薯产区晚疫病菌群体结构研究

为明确福建省部分马铃薯产区晚疫病菌群体结构,2017年-2019年在龙海市、福安市、霞浦县共分离获得96株马铃薯晚疫病菌。采用对峙培养法、鉴别寄主法和PCR-RFLP法对这些菌株的交配型、生理小种及线粒体DNA (mtDNA)单倍型进行分析。交配型测定结果显示,除了福建省福安市有5株(5.21%)为A1交配型外,其余91株(94.79%)均为自育型,未发现A2交配型及A1A2型菌株。从96个菌株中检测出16个生理小种类型,龙海市和福安市的优势生理小种均是可克服11个显性抗病单基因的1.2.3.4.5.6.7.8.9.10.11,霞浦县的优势生理小种为1.2.3.4.5.6.7.8.10。供试菌株均至少含有6个毒性基因。mtDNA单倍型共检测出3种类型,其中55个菌株(龙海市22株、福安市8株、霞浦县25株)为Ⅰa型,占57.29%,32个菌株(龙海市2株、福安市25株、霞浦县5株)为Ⅱa型,占33.33%,9个菌株(福安市7株、霞浦县2株)为Ⅱb型,占9.37%。研究结果表明,福建省马铃薯晚疫病菌群体遗传多样性日趋复杂。

Population structure of Phytophthora infestans isolates collected from a part of potato producing areas in Fujian province

To clarify the population structure of Phytophthora infestans in a part of potato producing areas in Fujian province,a total of 96 P.infestans isolates were collected from Longhai city,Fu’an city and Xiapu county of Fujian province from 2017 to 2019,and the mating type,physiological race and mitochondrial DNA haplotype were identified based on plate confrontation culture,host identification and PCR-RFLP.The results showed that 91 out of the 96 P.infestans isolates were self-fertile,accounting for 94.79%;five isolates collected from Fu’an belonged to A1 mating type,accounting for 5.21%,while A2 mating type and A1A2 type were not detected in this study.A total of 16 different physiological races were detected.The race 1.2.3.4.5.6.7.8.9.10.11 was the dominant species in Longhai and Fu’an cities,and the race 1.2.3.4.5.6.7.8.10 was the dominant species in Xiapu county.All of the isolates contained at least six virulence genes.Three mtDNA haplotypes(Ⅰa,Ⅱa andⅡb)were detected in this study,among which,Ⅰa type included 55 isolates(22 isolates in Longhai city,8 isolates in Fu’an city and 25 isolates in Xiapu county),accounting for 57.29%,Ⅱa type included 32 isolates(2 isolates in Longhai city,25 isolates in Fu’an city and 5 isolates in Xiapu county),accounting for 33.33%,andⅡb type included 9 isolates(7 isolates in Fu’an city and 2 isolates in Xiapu county),accounting for 9.37%.The results indicated that the composition of P.infestans populations was getting more and more complex in Fujian province.