羊肚菌菌盖干腐病病原菌鉴定及培养特性研究

菌盖干腐病是羊肚菌Morchella spp.出菇后发生最严重的病害之一,常给羊肚菌生产造成较大损失.为明确引起贵州六盘水地区羊肚菌菌盖干腐病致病菌种类,采用单孢分离法从六盘水地区3个羊肚菌栽培点的菌盖干腐病样品上分离到9个菌株.基于形态学及ITS和LSU基因序列分析将9个菌株鉴定为长孢卵单隔孢霉Diplo(o)spo...

Identification and cultural characterization of Diplo spora longispora associated with pileus rot disease on cultivated morel

Pileus rot disease widely occurs and often causes serious yield losses of cultivated morel.In order to identify the pathogen of pileus rot disease,nine strains were obtained by single-spore isolation in three cultivation sites of Liupanshui city.Based on morphological characteristics and ITS and LSU sequence analysis,the nine strains were identified as the same species,Diplo?spora longispora.Cultural characterization showed that PCA was the optimal medium for colony growth,the optimal nitrogen sources were peptone,ammonium nitrate,and the optimal carbon sources were glucose and maltose.The best temperature and initial pH ranges for spore germination and mycelial growth were 15-25℃and 6-10,respectively.When the temperature exceeded 30℃,the mycelium stopped growing and the spores failed to germinate.