| 羊肚菌菌盖干腐病病原菌鉴定及培养特性研究 |
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| 引用本文： | 黄 慧,张晓勇,郑 欢,蔡青苡,罗佳佳,罗 凯,李树江,杨友联.羊肚菌菌盖干腐病病原菌鉴定及培养特性研究[J].植物保护,2022,48(1):66-72. |
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| 作者姓名： | 黄 慧 张晓勇 郑 欢 蔡青苡 罗佳佳 罗 凯 李树江 杨友联 |
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| 作者单位： | 1. 六盘水师范学院生物科学与技术学院, 六盘水 553004; 2. 贵州省六盘水市农业科学院, 六盘水 553004 |
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| 基金项目： | 贵州省特色重点实验室建设项目(黔教合KY 字［2017］012);2019年贵州省大学生创新创业训练计划(20195201506);六盘水市科技计划(520202016HK07，52020-2018-03-07);2018年度六盘水师范学院项目“微生物学教学团队”(LPSSYjxtd201802) |
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| 摘 要： | 菌盖干腐病是羊肚菌Morchella spp.出菇后发生最严重的病害之一,常给羊肚菌生产造成较大损失.为明确引起贵州六盘水地区羊肚菌菌盖干腐病致病菌种类,采用单孢分离法从六盘水地区3个羊肚菌栽培点的菌盖干腐病样品上分离到9个菌株.基于形态学及ITS和LSU基因序列分析将9个菌株鉴定为长孢卵单隔孢霉Diplo(o)spo...
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| 关 键 词： | 羊肚菌 菌盖干腐病 长孢卵单隔孢霉 系统鉴定 培养特性 |
| 收稿时间： | 2020/10/13 0:00:00 |
| 修稿时间： | 2020/11/27 0:00:00 |
Identification and cultural characterization of Diplo spora longispora associated with pileus rot disease on cultivated morel |
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| HUANG Hui,ZHANG Xiaoyong,ZHENG Huan,CAI Qingyi,LUO Jiaji,LUO Kai,LI Shujiang,YANG Youlian.Identification and cultural characterization of Diplo spora longispora associated with pileus rot disease on cultivated morel[J].Plant Protection,2022,48(1):66-72. |
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| Authors: | HUANG Hui ZHANG Xiaoyong ZHENG Huan CAI Qingyi LUO Jiaji LUO Kai LI Shujiang YANG Youlian |
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| Institution: | 1. School of Biological Sciences and Technology, Liupanshui Normal University, Liupanshui 553004, China; 2. Liupanshui Academy of Agricultural Sciences, Guizhou Province, Liupanshui 553004, China |
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| Abstract: | Pileus rot disease widely occurs and often causes serious yield losses of cultivated morel.In order to identify the pathogen of pileus rot disease,nine strains were obtained by single-spore isolation in three cultivation sites of Liupanshui city.Based on morphological characteristics and ITS and LSU sequence analysis,the nine strains were identified as the same species,Diplo?spora longispora.Cultural characterization showed that PCA was the optimal medium for colony growth,the optimal nitrogen sources were peptone,ammonium nitrate,and the optimal carbon sources were glucose and maltose.The best temperature and initial pH ranges for spore germination and mycelial growth were 15-25℃and 6-10,respectively.When the temperature exceeded 30℃,the mycelium stopped growing and the spores failed to germinate. |
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| Keywords: | morel pileus rot disease Diplo spora longispora phylogenetic analysis cultural characteristics |
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