二氧化碳在华北大黑鳃金龟幼虫定位寄主植物根系中的作用

为探究二氧化碳(CO2)在华北大黑鳃金龟Holotrichia oblita幼虫定位寄主植物根系时所发挥的作用,对其开展了触角电位生理反应及行为生测试验.研究结果表明,CO2浓度为0.75％、1.5％、3％、6％时均能显著激发幼虫的触角电位和趋性反应,且随着浓度增加幼虫对CO2的趋向爬行所用时间显著缩短;根系分泌物同样...

The role of carbon dioxide in the orientation of Holotrichia oblita larvae to the host plant root

In order to explore the role of carbon dioxide (CO2) in locating the roots of host plants by the larvae of Holotrichia oblita, the electroantennogram (EAG) and behavioral responses were assayed. The results showed that the stimulation of 0.75%, 1.5%, 3%, 6% CO2 elicited a strong reaction of the larvae, and their crawling speed became faster with increasing concentration of CO2. The root exudates could also induce an EAG and chemotaxis response of H. oblita larvae, and the results were similar to that of the group of 1.5% CO2. Furthermore, mixing 1.5% CO2 with nine kinds of root exudates, i.e., tripalmitin, 2- butenoic acid, myristic acid, tetradecane, 4 methyl 2 oxovaleric acid, dodecane, inositol, o-xylene and p-hydroxybenzoic acid, respectively, could enhance the attraction effect and significantly shorten the crawling time. These results suggested that CO2 played an important role in guiding and enhancing the locating capability of the larvae of H. oblita for the host plant roots, which provides a new idea for green control of H. oblita larvae.