| 侵染大豆的花生斑驳病毒公主岭分离物基因组测序及分析 |
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| 引用本文： | 张春雨,李小宇,王 晨,战笑蕾,刘建青,王永志. 侵染大豆的花生斑驳病毒公主岭分离物基因组测序及分析[J]. 植物保护, 2022, 48(1): 61-65 |
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| 作者姓名： | 张春雨 李小宇 王 晨 战笑蕾 刘建青 王永志 |
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| 作者单位： | 1. 吉林省农业科学院, 公主岭 136100; 2. 吉林农业大学, 长春 130118; 3. 长春师范大学, 长春 130032 |
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| 基金项目： | 吉林省科技厅重点研发计划(20180201013NY)；吉林省农业科技创新工程(CXGC2021ZY022) |
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| 摘 要： | 本研究利用小RNA深度测序法在大豆叶片上检测到1株花生斑驳病毒Peanut mottle virus (PeMoV),根据小RNA深度测序结果和GenBank公布的PeMoV基因组序列设计引物克隆了PeMoV公主岭分离物(PeMoV-Gongzhuling)的基因组序列.测序结果经拼接后获得了PeMoV-Gongzhu...
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| 关 键 词： | 花生斑驳病毒 基因组 大豆 |
| 收稿时间： | 2020-10-16 |
| 修稿时间： | 2020-11-24 |
Sequencing and analysis of the genome of Peanut mottle virus (PeMoV) Gongzhuling isolate infecting soybean |
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| ZHANG Chunyu,LI Xiaoyu,WANG Chen,ZHAN Xiaolei,LIU Jianqing,WANG Yongzhi. Sequencing and analysis of the genome of Peanut mottle virus (PeMoV) Gongzhuling isolate infecting soybean[J]. Plant Protection, 2022, 48(1): 61-65 |
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| Authors: | ZHANG Chunyu LI Xiaoyu WANG Chen ZHAN Xiaolei LIU Jianqing WANG Yongzhi |
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| Affiliation: | 1. Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China; 2. Jilin Agricultural University, Changchun 130118, China; 3. Changchun Normal University, Changchun 130032, China |
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| Abstract: | In this study, the Peanut mottle virus (PeMoV) from soybean leaves was detected by small RNA deep sequencing. Primers were designed according to results of sRNA deep sequencing and the published genome sequences of PeMoV on GenBank, and the segments of genome of PeMoV Gongzhuling isolate (PeMoV Gongzhuling) was amplified by RT PCR. The assembled genome of PeMoV Gongzhuling was 9 709 nucleotides (GenBank accession no. MT790744). A large open reading frame (ORF) was found located between the 123th and the 9422th nucleotides, which encoded a polypeptide of 351.28 kD. By comparing it with other isolates of PeMoV, the nucleotide identity was 95.88%-99.53%, and the amino acid identity was 97.39%-99.84%, and it shared the highest identity with the South Korea isolate GYBU 92 (MT603819). |
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| Keywords: | Peanut mottle virus (PeMoV) genome soybean |
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