| 猪氟烷基因型的PCR检测及杂合子氟烷阳性猪的发现 |
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| 引用本文： | 刘铁铮,徐晓波,许康朴,葛云山,谢云敏.猪氟烷基因型的PCR检测及杂合子氟烷阳性猪的发现[J].中国兽医学报,1999,19(2):190-192. |
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| 作者姓名： | 刘铁铮 徐晓波 许康朴 葛云山 谢云敏 |
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| 作者单位： | 1. 江苏省农业科学院畜牧兽医研究所,南京,210014
2. 美国康奈尔大学医学院 |
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| 摘 要： | 对１４７头猪进行了氟烷测验，测得氟烷阳性猪３头，阳性率为２．０４％。运用ＰＣＲ－ＲＦＬＰ分析法对氟烷阳性猪及其同胞进行了基因型检测，结果４头被测猪中有２头为隐性纯合子，２头为杂合子。结合氟烷测验结果，２头隐性纯合子表现为氟烷阳性，符合一般规律；而２头杂合子中发现１头为氟烷阳性，此前尚未见报道，这一结果证明了氟烷表现与基因型并非完全对应
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| 关 键 词： | 氟烷测验 PCR－RFLP 杂合子 猪 |
| 修稿时间： | 1998-03-26 |
Detection of Halothane Genotype with PCR-RFLP and Discovery of Halothane Positive Pig with Heterozygote |
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| Liu Tiezheng,Xu Xiaobo,Xu Kangpu,Ge Yunshan,Xie Yunmin.Detection of Halothane Genotype with PCR-RFLP and Discovery of Halothane Positive Pig with Heterozygote[J].Chinese Journal of Veterinary Science,1999,19(2):190-192. |
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| Authors: | Liu Tiezheng Xu Xiaobo Xu Kangpu Ge Yunshan Xie Yunmin |
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| Abstract: | Halothane test (HT) was carried out on 147 pigs. Three pigs expressed halothane positive (HP) with the HP rate of 2.04%. The genotypes of HP pigs and their siblings (total 4 pigs) were detected with PCR RFLP. 2 of them were recessive homozygote (nn), and the other 2 were heterozygote (Nn). With the comparision of the HT and PCR RFLP, 1 of 2 Nn pigs was found to be HP, which had not been reported before indicating that there is no complete correspounding relationship between the phenotype and genotype of halothane. |
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| Keywords: | halothane test PCR RFLP heterozygote pig |
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