苹果属观赏海棠实生单株亲本的AFLP鉴定

应用AFLP技术, 对以苹果属观赏海棠品种王族(Malus ‘Royalty’) 为母本, 以5个材料为父本的38个实生单株进行多态性分析。结果表明: 筛选出的9对分辨率高的引物共扩增出349条带, 其中多态性带303条, 多态位点百分率达86.8%。根据聚类分析和电泳图谱扩增得到亲本材料的特征带, 鉴定出19株实生单株的父本可能为‘宝石’ (Malus‘Jewelberry’) ; 7株实生单株的父本可能为海棠花(Malus spectabilis Borkh. ) ; 3株实生单株的父本可能为草莓果冻(Malus‘Strawberry Parfait’) ; 不能确定其父本的实生单株有9株。

Identification of Parentage of Ornamental Crabapple Seedlings Using AFLP Markers

The polymorphism was analyzed by AFLP in 38 Ornamental Crabapple seedlings from female parent, Malus ' Royalty' , and 5 possible male parents. Results showed that the total 349 informative AFLP fragments were generated using the selected 9 EcoR I -Mse I primer combinations with high resolution capacity. Among them there were 303 polymorphic fragments with a polymorphism percentage of 86.8%.According to the characteristic bands of electrophoretogram and Cluster Analysis, the male parents of 19, 7 and 3 seedlings were identified to be Malus 'Jewelberry' , Malus spectabilis Borkh. and Malus 'Strawberry Parfait' respectively. The remnant 9 seedlings could not be confirmed or identified.