| 乌桕优选单株的离体快速繁殖技术研究 |
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| 引用本文： | 蒋泽平,倪竞德,张敏,董筱昀,李谊忠.乌桕优选单株的离体快速繁殖技术研究[J].江苏林业科技,2011,38(5):7-10. |
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| 作者姓名： | 蒋泽平 倪竞德 张敏 董筱昀 李谊忠 |
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| 作者单位： | 1. 江苏省林业科学研究院,江苏南京,211153
2. 镇江南山农艺有限公司,江苏镇江,221211 |
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| 基金项目： | 江苏省科技支撑农业计划项目“彩叶乌桕乡土景观植物的选育”(BE2009323); 镇江市农业科技支撑计划项目“江苏省丘陵地区乡土能源树种种质资源库建设与快繁技术研究(NY20100024)研究内容之一 |
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| 摘 要： | 选择8年生乌桕优树母株，取其枝条的腋芽茎段为外植体，进行组织培养快速繁殖技术研究。结果表明，接种时，先用70％的酒精对带芽茎段消毒30s，再用0．1％升汞灭菌11min，污染率可降到18．5％。在初代培养中，芽诱导最佳培养基为WPM＋6-BA1．5mg／L＋NAA0．2mg／L，30d后的诱导率可达85．4％。在4／5...
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| 关 键 词： | 乌桕 优株 组织培养 |
In vitro culture and plant regeneration of Sapium sebiferum |
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| JIANG Ze-ping,NI Jing-de,ZHANG Min,DONG Xiao-yun,LI Yi-zhong.In vitro culture and plant regeneration of Sapium sebiferum[J].Journal of Jiangsu Forestry Science & Technology,2011,38(5):7-10. |
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| Authors: | JIANG Ze-ping NI Jing-de ZHANG Min DONG Xiao-yun LI Yi-zhong |
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| Institution: | JIANG Ze-ping1,NI Jing-de1,ZHANG Min1,DONG Xiao-yun1,LI Yi-zhong2(1.Jiangsu Academy of Forestry,Nanjing 211153,China,2.Zhenjiang Nanshan Agricultural Company limited,Zhenjiang 212000,China) |
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| Abstract: | The stem sections with axillary bud of Sapium sebiferum(L.) Roxb.(8 years old) were used as explants for the research of tissue culture and rapid propagation.The results showed that the contamination rate was 18.5% when we sterilized the explants with ethanol(70%) 30 seconds then used HgC12(0.1%) to sterilize the stem sections 11 minutes.The medium suitable for induction in primary culture was WPM+6-BA 1.5 mg/L+NAA 0.2 mg/L,and the inductivity reached to 85.4% after 30 days.The medium suitable for secondary... |
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| Keywords: | Sapium sebiferum Elite maternal plant Tissue culture |
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