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Plant recovery from seedling-derived shoot tips ofFaidherbia albida grown in vitro
Authors:T. J. Ruredzo  J. Hanson
Affiliation:(1) International Livestock Centre for Africa, P.O. Box 5689, Addis Ababa, Ethiopia;(2) Present address: SADC Regional Gene Bank, Private Bag CH6, ZA-153 02 Lusaka, Zambia
Abstract:In vitro cultures were initiated from shoots taken from seedlings ofFaidherbia albida on Murashige and Skoog based medium supplemented with different combinations of 6-benzylaminopurine (BA) and agr-naphthaleneacetic acid (NAA). The shoots grew and rooted on all media. Rooting and vigorous growth were most successful on medium supplemented with 10–7M NAA alone on which 87% of the shoots formed roots. Seventy-one percent of plantlets which were transferred to soil were successfully established and nodulated with the nativeRhizobium. The procedures provide a basis for the development of in vitro techniques for rapid multiplication and physiological studies of the species.
Keywords:Faidherbia albida  Acacia albida  tissue culture  in vitro culture  biotechnology
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